Growth arrest-specific 6 (GAS6) protein plays a key role in processes related to proliferation, inflammation, angiogenesis, and atherosclerotic plaque formation. In addition, it has been reported that plasma levels of GAS6 are cancer prognosis other relevant pathologies, such as heart failure or sepsis. We report here the first electrochemical immunoplatform for determination GAS6, which demons...

Herein, we report preparation of magnetic hybrid nanoflower (MhNF) formed horseradish peroxidase (HRP), copper ions (Cu2+) and iron oxide nanoparticles (Fe3O4 NPs) with greatly enhanced activity, stability, reusability applicability for use in removal phenol from aqueous solution. In this study, pre-synthesized small sized Fe3O4 NPs were immobilized to synthesized HRP-Cu2+ (hNFs). The MhNFs cha...

Soluble epoxide hydrolase (sEH) is a potential pharmacological target for treating hypertension, vascular inflammation, cancer, pain, and multiple cardiovascular related diseases. A variable domain of the heavy chain antibody (termed single domain antibody (sdAb), nanobody, or VHH) possesses the advantages of small size, high stability, ease of genetic manipulation, and ability for continuous m...

A sensitive and versatile competitive enzyme immunoassay (cEIA) has been developed for the quantitative detection of cocaine in complex forensic samples. Polyclonal anti-cocaine antibody was purified from serum and deposited onto microtiter plates. The concentration of the cocaine antibody adsorbed onto the plates, and the dilution of the cocaine-HRP hapten were both studied to achieve an optim...

Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red the most widely used substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular improved sensitivity and selectivity HRP thus desired. Herein, three dihydroflu...

To design an enzyme immunoassay-based method, the most important is conjugation of enzyme with antigens or antibodies and stability of labeled conjugate. In this study, human albumin was conjugated with horseradish peroxidase using periodate oxidation method. To stabilize biological activity and functional structure of different components of protein conjugate, special storage conditions are re...

Bisphenol A (BPA) is a commonly used material made of polycarbonate, which is used in food containers. BPA is known to be an endocrine disruptor. To investigate the effect of BPA on health, animal experiments are necessary. To determine the intake of BPA in food or drink by animals, a BPA-sensing system that can detect a small amount of BPA in biological samples within a short reaction time is ...

An ultrasensitive portable electrochemical immunosensor for human immunodeficiency virus p24 (HIV p24) antigen detection has been developed, whereby the detection sensitivity was 1000 times higher than that of the ELISA method. Firstly, a novel HRP enzyme-antibody copolymer (EV-p24 Ab2) was synthesized through an EnVision regent (EV, a dextrin amine skeleton anchoring more than 100 molecules of...

An emerging trend in utilizing service robots a vast range of application areas could be seen nowadays as promising effort to uplift the living standard. These are intended used by non-expert users, and their tasks often require navigation human-populated environments. Thus, human-friendly behavior is expected from these users. A robot should aware Human–Robot Proxemics (HRP) facilitate behavio...

The binding behavior of a competitive inhibitor, N-phenylbenzhydroxamic acid (BHA) against horseradish peroxidase (HRP) was studied in order to understand and predict the interaction mechanism of hydrogen donors with the enzyme. The dissociation constants of the complexes of HRP-BHA, HRP-donor and HRP-BHA-azide were estimated at specified conditions by difference spectroscopy. The binding s...