ABSTRACT Artesunate (ART) is a readily available anti malarial in combination therapy. The assay method has posed a challenge because it does not have a readily recognisable absorption chromophore needed for UV spectroscopy. A simple and rapid assay method involving two reaction steps has been developed for assay of ART in pharmaceutical formulations. The method involves basic reaction of ethan...

Blood HbA1c determination is a powerful tool for the evaluation and management of patients with diabetes mellitus. Many HbA1c analytical methods demonstrate bias in samples from patients with hemoglobinopathies. This study evaluated the analytical performance of Roche Diagnostics' 1st and 2nd generation HbA1c assays in patients with or without hemoglobinopathies whose HbA1c levels were elevated...

Acylcarnitines are formed by esterification between fatty acids CoA or organic acids CoA molecules and carnitine. In various fatty acids oxidation defects and organic acidurias, there is increased concentration of corresponding acylcarnitines. Abnormalities in specific acylcarnitines are used in the diagnosis of fatty acids oxidation defects and organic acidurias. Most commonly used method for ...

The activity of liver microsomal CYP2E1 is commonly measured as the rate of 5-chloro-2-benzoxazolone (chlorzoxazone) 6-hydroxylation, which requires separation of 6-hydroxychlorzoxazone and chlorzoxazone by high pressure liquid chromatography (HPLC). In the present study, we describe a solvent extraction (non-HPLC) assay for measuring CYP2E1 activity, based on the 6-hydroxylation of [14C]chlorz...

A simple, precise, specific ,robust and rapid HPLC assay for the of measurement meloxicam in pharmaceutical formulations was developed and validated at Department of Pharmacy and Central Quality Assurance Laboratory, LCWU, Lahore. The solution of meloxicam standard was prepared in HPLC grade acetonitrile (5 μg.mL) and ultra sonicated for 15 minutes. One mL sample mixed well with one mL of HPLC ...