Through the kindness of Dr. W. H. Park we have been enabled to study a horse antipoliomyelitic serum. This preparation has been supplied us in three forms: citrated blood plasma, serum, and globulin concentrate. We have tested these preparations in vitro and in vivo for inactivating or neutralizing or, to use perhaps a better term, antiviral effects against a constant, potent, filtrate virus of...

The degree of histological deterioration of the original explant and the extent of cell spreading was evaluated in cultures of pectoralis muscle from 11-day chicks. Although the frequencies of these two parameters varied with the amounts of horse serum and embryo extract added to the medium, cultures from dystrophic chicks, in comparison to those from either normal or heterozygous animals, cons...

1. Antibody produced in the horse migrates as a new serum component between the beta and gamma components, whereas rabbit antibody is electrophoretically identical with the gamma globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the gamma globulin fraction can be determined by integration of the electrophoresis diagrams of unabsorbe...

John, T. Jacob (University of Colorado School of Medicine, Denver), Marlene Stahl, and Vincent A. Fulginiti. Cumulative hemagglutination by Mycoplasma pneumoniae and other agglutinins. J. Bacteriol. 92:1002-1004. 1966.-The phenomenon of cumulative hemagglutination, or hemagglutination by two agglutinins, each in subagglutinating concentration, was demonstrated by use of four different systems, ...

Absorption: With the original technique (1) the horse serum decomplemented by heating to 56 0 C. and then absorbed against cells and pooled serum of the species to be treated; tion was done first to avoid hemolysis of the added red cedure had two flaws. First, the use of serum (rather than p.,,"~ ... aJ ICtJ,:'lgror absorption precluded the removal of antifibrinogen and bocyte antibodies. Secon...

In mammal circulation, various ferritin-binding proteins (FBPs) are thought to be involved in the clearance of circulating ferritin after complex formation with it. However, horse FBPs are known to cause inhibitory effects on ferritin immunoassay due to the concealment of the ferritin molecule to anti-ferritin antibodies used in the ferritin immunoassay. These inhibitory effects are eliminated ...

Pyrilamine (mepyramine) is an H1-receptor antagonist used in human and veterinary medicine. It has the potential to produce central nervous system effects in horses and therefore may have some impact on an outcome of a horse race. A single oral dose of pyrilamine (300 mg/horse) was given to three animals. Serum samples were collected before drug administration and at 0.25, 0.5, 1, 2, 4, 6, 24, ...

1. The addition of small amounts of cholesterol and of cephalin reduces markedly the protective action of antipneumococcus horse serum. 2. These lipids do not affect the protective action of antipneumococcus rabbit serum. 3. These findings may be explained (a)by the selective adsorption of lipid on the antigen-antibody complex, and (b) by certain lipid antagonisms. 4. The failure of large amoun...

1. Data are presented showing that precipitin tests can be used for the detection of the proteins contained in egg white and horse serum in the blood stream and thoracic duct lymph following injection into the knee joints of normal dogs. The sera of rabbits immunized against the particular protein employed were used in doing the precipitin tests. 2. Egg white is removed only by way of the lymph...

BACKGROUND The therapeutic efficacy of horse antilymphocyte globulins (ALG) or of rabbit antithymocyte globulins (ATG), used for both the prevention and treatment of allograft rejection has been well documented. However, clinical use of these heterologous antibodies can result in the production of antibodies against horse or rabbit proteins and in the development of serum sickness via circulati...