A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

in this paper, construction rules of the root locus of a positive feedback system whose loop transfer function is not strictly proper have been investigated. the root locus method is being used since 1950. it is demonstrated that some of the steps in this technique are not general and for a positive feedback system whose loop transfer function is not strictly proper are not valid. to overcome t...

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

Case I.?Sowar Bhart Singh, aged 26, Rajput, on 9th August, while acting as a beater, was attacked by a wounded tigress. His matchlock missed fire; he struck one blow on the head with his talwar, and was then knocked down. One of the officers with ?whom he had gone out, shot the tigress through the head while she was standing over the sowar. He was carried in 15 miles, and I saw him at 10 o'cloc...

Nodare Chand, aped CO years, a Hindoo goldsmith was admitted on the 13th February, 1867, with an impetiginous eruption on one-half of the face, lie stated that about ei"-ht days ago whilst blowing a fire through a bamboo tube, a spark flew into the external corner of his left eye. Next morning the face swelled, and two days later the left side of his face was covered with an eruption, but he co...

At that time he was passing through his first attack of acute mania, having been ill three months. He was not suicidal, but was dangerous to others. His family history, physical and psychological, was good, there being no history of phthisis, of neuroses, or insanity. His own bodily condition was good, and he was free from any physical ailment : he was not ruptured on admission. Mentally, he wa...