We studied gamma-aminobutyric acid (GABA)-mediated currents in short-term cultured dorsal unpaired median (DUM) neurons of cockroach Periplaneta americana using the whole cell patch-clamp technique in symmetrical chloride solutions. All DUM neurons voltage-clamped at -50 mV displayed inward currents (I(GABA)) when 10(-4) M of GABA was applied by pneumatic pressure-ejection pulses. The semi-loga...

An experiment was carried out to assess the effect of four nitrogen levels viz. T1 (full doze of urea i.e. 215 kg urea ha-1 at 15 DAT), T2 (full doze of urea at two equal splits, ½ at 15 DAT + ½ at 30 DAT), T3 (full doze of urea at two equal splits, ½ at 15 DAT + ½ at 55 DAT) and T4 (full doze of urea at three equal splits, 1/3 at 15 DAT + 1/3 at 30 DAT + 1/3 at 55 DAT) on morpho-physiological ...

Nanocrystalline mixed metal oxides (MMO) of various metal cations were synthesized and were used for coating a piece of copper wire as a new high sensitive solid phase micro extraction (SPME) fiber in extraction and determination of BTEX compounds from the headspace of aqueous samples prior to GC-FID analysis. Under optimum extraction conditions, the proposed fiber exhibited low detection limit...

to assess nickel-induced toxicity in plants, zea mays seeds were germinated and cultured on nutrient solution with nickel concentrations of 0, 50, 100 and 200 μm for a period of two weeks. its effects on the growth, hill reaction and photosynthetic pigments content were then investigated. the fresh and dry weight of leaves and roots increased in 50 μm nickel, but decreased in 100 and 200 μm. th...

“IC is the current in drug free solution obtained by averaging the current before and after application, IC50 is the drug concentration at which the inhibition is half maximal, h is the Hill coefficient, L is a scaling factor reflecting the difference between channel activity in control and MgATPcontaining solution (L = 1 in the absence of the nt) and a is the fraction of KATP current remaining...

Dose-response (or 'concentration-effect') relationships commonly occur in biological and pharmacological systems and are well characterised by Hill curves. These curves are described by an equation with two parameters: the inhibitory concentration 50% (IC50); and the Hill coefficient. Typically just the 'best fit' parameter values are reported in the literature. Here we introduce a Python-based...