background: genes for human epidermal growth factor receptors b1 (erbb1) and b2 (erbb2) were amplified in breast and ovarian cancers. both of them were associated with aggressive disease and worse prognosis. the erbb1 or erbb2 status of a tumor may provide an indication of the response to erbb1 and erbb2 -targeted therapies. for accurate and rapid assessment of amplification of erbb1 and erbb2 ...

Temperature-Controlled Microchip Liquid Chromatography System Thesis by Victor Chi-Yuan Shih Doctor of Philosophy in Electrical Engineering California Institute of Technology High-performance liquid chromatography (HPLC) is one of the most important analytical tools heavily used in the fields of chemistry, biotechnology, pharmaceutics, and the food industry. The power of liquid chromatography c...

separation of ten sedative- hypnotic drugs was performed by rp-hplc using mixed micellar mobile phase. effect of temperature, type and amount of organic modifier in mobile phase on efficiency (n) and asymmetry factor (b/a) showed that, the appropriate conditions for a good separation were 35°c and 7% (v/v) butanol in mobile phase.  variations of selectivity factor versus butanol concentration, ...

Overview Purpose: To examine the use of targeted principal component analysis (PCA) with HPLC-charged aerosol detector data as a means of determining adulteration of olive oils. Method: Oil samples were analyzed as both untreated and hydrolyzed, using a C30 HPLC method and charged aerosol detection. PCA results were determined using peak area percent values of the acylglycerides and free fatty ...

Hazelnuts were investigated for the presence of antioxidant compounds other than tocopherol. Hazelnuts from 2 regions were extracted with methanol:water (2:1 ; v/v). Extracts were subjected to either acid or alkaline hydrolysis but neither improved the separation quality during high pressure liquid chromatography (HPLC) analysis. Nonhydrolyzed extracts of hazelnut had greater antioxidant activi...

separation of some biogenic amines via rp-hplc using mixed micellar mobile phase was investigated. the compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (sds and brij-35) and the ph of the mobile ...