We compared the results obtained by a micromethod for the determination of plasma lipoprotein cholesterol, in which electrophoresis is used to separate the lipoprotein fractions (beta-, pre-beta-, and alpha-lipoproteins), with those determinations with ultracentrifugation (low-density, very-low-density, and high-density lipoproteins). Precision of determination (coefficient of variation, CV, %)...

The role of insulin in the regulation of lipoprotein cholesterol distribution was studied in 18 human volunteers who were of normal weight, normolipemic, and glucose-tolerant. We used the euglycemic clamp technique and made comparisons with six saline-infused controls. While total cholesterol and low density lipoprotein cholesterol levels fell similarly by 6 hours in both groups (p = NS), there...

Our specific aim in this study was to assess the family resemblance for lipids and lipoproteins in blacks randomly selected from the Princeton School District's Family Study cohort, and compare the family resemblance of lipids and lipoproteins between the blacks and whites from the same cohort. There were 160 white and 59 black nuclear families ascertained through randomly selected family study...

0

We report development of a model of retroviral gene transduction in high-density limb bud cell micromass culture. The replication competent avian retrovirus RCAS BP (A) carrying the human placental alkaline phosphatase gene (RCAS AP) was used as a marker for retroviral infection and spread. The final protocol balances the need to allow time for retroviral integration and gene transduction again...

Changes in serum triglyceride and high density lipoprotein (HDL) cholesterol after a fatty meal have been studied in smokers and nonsmokers. Average serum triglyceride during the study was higher in smokers than in non-smokers. In non-smokers there was a rise in the HDL2/HDL3 cholesterol ratio after oral fat, but not in smokers. These findings are compatible with the hypothesis that smoking int...

We calculate the phase diagram of amorphous solid water by performing molecular dynamics simulations using the extended simple point charge (SPC/E) model. Our simulations follow different paths in the phase diagram: isothermal compression/decompression, isochoric cooling/heating, and isobaric cooling/heating. We are able to identify low-density amorphous (LDA), high-density amorphous (HDA), and...