نتایج جستجو برای: hi assay

تعداد نتایج: 236455  

Journal: :Journal of clinical microbiology 1998
F Bouche W Ammerlaan F Berthet S Houard F Schneider C P Muller

Recombinant hemagglutinin (H) protein of the measles virus (MV) was produced in mammalian cells with a high-yield expression system based on the Semliki Forest virus replicon. Crude membrane preparations of H protein-transfected BHK-21 cells were used to coat microtiter plates to measure specific immunoglobulin G antibodies in 228 serologically defined serum samples mainly from measles late-con...

2015
Eun-Ju KIM Bo-Hye KIM Sunjoo YANG Eun-Jin CHOI Ye-Jin SHIN Jae-Young SONG Yeun-Kyung SHIN

In this study, antibody responses after equine influenza vaccination were investigated among 1,098 horses in Korea using the hemagglutination inhibition (HI) assay. The equine influenza viruses, A/equine/South Africa/4/03 (H3N8) and A/equine/Wildeshausen/1/08 (H3N8), were used as antigens in the HI assay. The mean seropositive rates were 91.7% (geometric mean antibody levels (GMT), 56.8) and 93...

2007
Nigel J. Temperton Katja Hoschler Diane Major Carolyn Nicolson Ruth Manvell Vo Minh Hien Do Quang Ha Menno De Jong Maria Zambon Yasuhiro Takeuchi Robin A. Weiss

BACKGROUND The World Health Organisation (WHO) recommended the development of simple, safe, sensitive and specific neutralization assays for avian influenza antibodies. We have used retroviral pseudotypes bearing influenza H5 hemagglutinin (HA) as safe, surrogate viruses for influenza neutralization assays which can be carried out at Biosafety Level 2. RESULTS Using our assay, sera from patie...

B Heydarchi, M Tavassoti-Kheiri, A Torabi , F Fotouhi , M Tabatabaiean , M Taghizadeh , SF Mousavi , SM Hosseini ,

Background and Aims: Influenza vaccination is one of the best way to prevent and control influenza worldwide. It is manufactured by WHO-licensed companies based on the WHO expertise committee annually. The aim of this study was partial quality control of the commercial human influenza vaccine 2008-9 and its matching with the circulating strains. Materials and Methods: The trivalent imported vac...

Journal: :The Journal of veterinary medical science 2010
Jinliang Zhang Yan Guo Yuncai Xiao Xiliang Wang Zhonghua Li Sishun Hu Dingren Bi

A simple gold-immunochromatographic assay (GICA) based on indirect reaction format was developed for rapid detection of antibodies against avian infectious bronchitis virus (IBV). The detection time of IBV IgG by the GICA was less than 10 min, whereas the HI test and the enzyme-linked immunosorbent assay (ELISA) require 1-2 hr. Reference sera against newcastle disease virus, infectious bursal d...

Journal: :Human molecular genetics 2008
Teruki Yanagi Masashi Akiyama Hiroshi Nishihara Kaori Sakai Wataru Nishie Shinya Tanaka Hiroshi Shimizu

Harlequin ichthyosis (HI), which is the most severe genodermatosis, is caused by loss-of-function mutations in ABCA12, a member of the ATP-binding cassette transporter family. To investigate the pathomechanism of HI and the function of the ABCA12 protein, we generated ABCA12-deficient mice (Abca12(-/-)) by targeting Abca12. Abca12(-/-) mice closely reproduce the human HI phenotype, showing mark...

2011
Ben M. Hause

Materials and methods: The hemagglutinin genes from 37 δ-cluster SIV isolates were fully sequenced. The isolates were antigenically categorized using a high throughput serum neutralization (HTSN) assay incorporating antisera generated against the four currently circulating clusters of H1 SIV and a commercial vaccine containing a δ-cluster SIV isolate. A subset of the isolates were further chara...

Journal: :Neuro endocrinology letters 2014
Miroslav Pohanka

OBJECTIVES HI-6 or asoxime in some sources is an antidotum for nerve agents. In recent experiments, implication of HI-6 in immunity response was proved; however, the issue was not studied in details. In this experiment, role of cytokines in HI-6 impact on immunity was searched. DESIG N: BALB/c mice were exposed to saline, HI-6 in a dose 1-100 mg/kg and/or 1 keyhole limpet hemocyanin (KLH) 1 mg/...

Journal: :Veterinary microbiology 2013
Kozue Hotta Hiroki Takakuwa Toshiyo Yabuta Trang T H Ung Tatsufumi Usui Hang L K Nguyen Thanh T Le Mai Q Le Tsuyoshi Yamaguchi Koichi Otsuki Toshihiro Ito Toshiyuki Murase Tetsu Yamashiro

In Vietnam, numerous surveillance programs are conducted to monitor the prevalence of avian influenza (AI) viruses. Three serological methods-the agar-gel immunodiffusion test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay-are well established for detection of AI virus antibodies in poultry sera. Several recent reports have validated egg yolk as an alternative sou...

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