Using agro-infiltration technique, we have transiently expressed human Growth Hormone (hGH) in tobacco(Nicotiana tobacum), potato (Solanum tuberosum) and lettuce (Lactuca sativa) leaves. Out of three differentinoculation times used for infiltration in our study, it was seen that highest level of hGH expression wasachieved when leaves were infiltrated for 35 min. The presence o...

A 10-year-old child with idiopathic growth hormone deficiency was treated with recombinant methionyl human growth hormone (m-hGH, Somatonorm) at a dose of 14 IU/week. Height increased from 122.3 to 126.5 cm during the first 9 months of treatment (5.6 cm/y), but only from 126.5 to 126.6 cm during the next 3 month of treatment (0.4 cm/y). Anti-hGH antibody was detected at 2 months of treatment, r...

Human growth hormone (hGH) gene expression and regulation by thyroid and glucocorticoid hormones were examined after its stable introduction into rat anterior pituitary tumor cells. Transcripts from the hGH gene were correctly initiated and could be positively regulated by glucocorticoids. In contrast to the endogenous rat GH gene and a transfected, modified rat GH gene, the hGH gene was negati...

ACONSEQUENCE OF THE COMMITTEE ON OVERsight and Government Reform’s recent hearing on the Mitchell Report on steroid and growth hormone (hGH) use was recognition that the misguided use of hGH by professional athletes and entertainers contributes to the far more frequent and increasing problem of anti-aging clinics, Web sites, compounding pharmacies, and producers selling the drug to the general ...

Human growth hormone (hGH) gene deletion (6.7 to 7.6 kb) is one of the causes of isolated growth hormone deficiency (IGHD), named IGHD IA. IGHD IA, however, only accounts for about 10% of the total IGHD patients. Most IGHD is caused by unknown mechanisms. Here, hGH gene 5' sequences in three IGHD patients without hGH gene deletion were analysed to see if there was any mutation hindering the exp...

The effects of administered human growth hormone (HGH) were evaluated in dwarfed, prepubertal children who were receiving long-term corticosteroid therapy for a chronic disease. During 11 complete metabolic balance studies, the eight corticosteroid-treated children demonstrated impaired response to large doses of HGH with minimal nitrogen and no phosphorus retention. In contrast, two hypopituit...

growth hormone (HGH) levels was investigated in normal young male subjects. The administration of nicotinic acid with its concomitant lowering of plasma FFA was followed by a rise of plasma HGH. The HGH rise could be inhibited by the simultaneous administration of heparin which prevented the fall of plasma FFA, or by glucose administration. The administration of heparin alone resulted in an inc...

Recombinant human growth hormone (r-hGH) can be produced by recombinant Escherichia coli a main host for recombinant protein productions. In order to improve the productivity of r-hGH, an orthogonal (L 16 (4×2)) experiment was applied to optimize the best culture conditions for r-hGH production by flask cultures of E. coli BL (21) harboring a new constructed plasmid (pEHUb-hGH). Based on the re...

The death rates of hepatocellular carcinoma (HCC) are extremely high due to the paucity of therapeutic options. Animal models and anecdotal clinical evidence indicate a potential role of hGH and hPRL in HCC. However, the prognostic relevance and the functional role of tumor expression of these hormones in human HCC are not defined. Herein, we analyzed the mRNA and protein expression of hGH and ...