A new compound, 1,3,7,9-tetrahydroxy-6 H-dibenzo[ b, d]pyran-6-one (1), together with 6 known compounds (2-7), was isolated from the extract of whole plants Centella asiatica. The chemical structure elucidated on basis spectroscopic and mass spectrometric analyses. All isolates were evaluated for their cytotoxicities against 4 human cancer cell lines (MCF-7, HepG2, Hela, A549). Among them, comp...

Hepatitis C virus (HCV) is a significant health problem facing the world. More than 170 million people are infected with HCV worldwide. HCV encodes a large polyprotein precursor that is processed into at least 10 distinct products including structural (core, E1 and E2) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B). Besides its importance in virus replication, NS4A functions as a cofa...

background hepatocytes are used as an in vitro model to evaluate drug metabolism. human hepatocyte transplant has been considered as the temporary treatment of acute liver failure. optimization freezing methods is very important to preserve both cell viability and function which are achieved by cryopreservation mostly always. objectives the present study aimed to investigate the cryoprotective ...

The toxicity of CuO nanoparticles (CuO NPs) to human hepatocarcinoma (HepG2) cells was investigated in this study. CuO NPs (1 40 mg/L) had significant toxicity to HepG2 cells. The antioxidant N-acetyl-L-cysteine (NAC) significantly reduces the cytotoxicity induced by the CuO NPs, supporting the hypothesis that oxidative stress contributes to the cytotoxicity of CuO NPs. To further explore the o...

BACKGROUND Hepatocellular carcinoma (HCC) is the most important cause of cancer-related deaths worldwide. Pirfenidone is an orally available small molecule with therapeutic potential for fibrotic diseases. MATERIAL AND METHODS In this study, we analyzed the effects of different pirfenidone concentrations on the proliferation of HepG2 HCC cells using Cell Counting Kit-8 (CCK-8) and colony format...

The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines. REC-2006 treatment 2 h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells. The expression of nuclear apoptosis inducing factor (AIF) was found to be more inhibited (~17%) in HepG2 cells in REC-2006 + radiation-treated gro...

Multidrug resistance (MDR) is one of the major reasons for the failure of liver cancer chemotherapy, and its suppression may increase the efficacy of chemotherapy. NANOG plays a key role in the regulation of embryonic stem cell self-renewal and pluripotency. Recent studies reported that NANOG was abnormally expressed in several types of tumors, indicating that NANOG is related to tumor developm...

Although various in vitro assays have been developed to evaluate the cytochrome P450 (CYP)-inducing potential of drug candidates, there is a continuing need for the development of a reliable model in drug discovery. The objective of the present study was to compare CYP induction by chemicals in HepG2 cells with Huh7, NKNT-3, and reverted NKNT-3 cells. HepG2 cells showed more similarity to human...

A multivalent galactosyl carborane derivative 10 (dendritic glyco-borane, DGB) was synthesized and demonstrated as a potential cell-targeting agent in BNCT with HepG2 cells. DGB 10 improved the delivery of boron to HepG2 cells and neutron irradiation data show DGB 10 with ten-fold improvement at killing the HepG2 cells over BSH.