Clotting is critical in limiting hemolymph loss and initiating wound healing in insects as in vertebrates. It is also an important immune defense, quickly forming a secondary barrier to infection, immobilizing bacteria and thereby promoting their killing. However, hemolymph clotting is one of the least understood immune responses in insects. Here, we characterize fondue (fon; CG15825), an immun...

Natural hemagglutinin activity against vertebrate erythrocytes is present in the hemolymph of the cockroach Blabarus craniifer. The hemagglutinin titer against rabbit erythrocytes is high, whereas sheep and horse red cells agglutinate weakly. Hemagglutinin activity was depressed by the complement inhibitor, cobra venom factor. Cockroach hemagglutinin is heat-labile; all activity is destroyed by...

Lobsters and other crustaceans do not have sterile hemolymph. Despite this, little is known about the microbiome in hemolymph of lobster Homarus americanus . The purpose this study was to characterize lobsters. lobsters were part a larger on effect temperature epizootic shell disease, several died during course study, providing an opportunity examine differences microbiomes between live recentl...

Objective: The potential of marine crabs as a source of bioactive products is largely unexplored. Methods: Portunussegnis crabs were collected from the northern coast of Persian Gulf. Antimicrobial activities of hepatopancreas, hemolymph and proteins of hemplymph from P. segnis crabs against a range of 11 different bacterial and 6 fungal pathogen strains were examined. Two positive controls Amo...

l. After injection of NaCl into the abdominal hemocoel of BIaberus craniifer, the osmotic concentration of antenna1 hemolymph peaked at 1.25 hr and returned to pre-injection concentration by 21 hr. The mean plasma clearance was 2.67 @/hr. 2. Force-feeding of water 15min after injection of NaCl prevented the early rise in hemolymph osmotic concentration, but a later gradual increase in osmotic c...

The study carried out a 15-day feeding trial of the fleshy shrimp broodstock that have just finished their first spawn using five different maturation feed types (three manufactured feed, fresh and commercial feed) to induce multiple spawning in then analyzed compared hemolymph levels total protein, lipid, hemocyanin, glucose, cholesterol as well ovary histology between treatments identify pote...

The Pacific white shrimp, Litopenaeus vannamei, acclimated to 30 ppt salinity, was transferred to either low (15 and 5 ppt), or high (45 ppt) salinity for 7 days. Hemolymph osmolality, branchial carbonic anhydrase activity, and total ninhydrin-positive substances (TNPS) in abdominal muscle were then measured for each condition. Hemolymph osmotic concentration was regulated slightly below ambien...

A lectin was purified from the hemolymph of Sarcophaga peregrina larvae, obtained after injury of their body wall. This lectin agglutinated sheep red blood cells markedly and the hemagglutinating activity was inhibited by galactose and lactose. The active lectin was found to have a molecular weight of 190,000 and to consist of four alpha subunits and two beta subunits, with molecular weights of...

The total and dry body weight treated larvae of Aedes aegypti was significantly decreased at different time intervals under investigation (6, 12, 24, and 48 hrs) post-treatment with LC30 of Bt subsp. Kurstaki HD-1. The body water content was decreased significantly at 6 hrs post-treatment, while it was highly significant at the three other treatments. Also hemolymph volume was significantly dec...

Biosynthesis of locust apolipophorin-III (apo-III) was studied in vitro. Gel electrophoresis and immunoblotting analyses of the locust hemolymph demonstrated that apo-III first appears in the hemolymph on the day 3 of the adult stage after the final molt and its hemolymph concentration increases thereafter. When incubated in vitro in a medium containing radioactive amino acid, the fat body cell...