نتایج جستجو برای: hemi nested rt

تعداد نتایج: 92535  

دکتر تقی طریحی, , دکتر سید علیرضا مصباح, , فرزانه رهمدانی, ,

  چکید ه   سابقه و هدف   آنتی‌ژن RhD در ایجاد بیماری‌های همولیتیک نوزادان نقش مهمی دارد و از آن‌جایی که تعیین RhD جنین با استفاده از روش‌های تهاجمی مانند آمنیوسنتز، خطراتی را برای مادر و جنین به دنبال خواهد داشت، در این پژوهش تلاش گردید تا ژنوتایپ RhD جنین از DNA آزاد جنینی موجود در سرم مادران باردار، به صورت غیر تهاجمی و با استفاده از روش Hemi-nested PCR تعیین گردد.   مواد وروش‌ها   مطالعه انج...

Fadyia Mahdi Alameedy, Ghaida Raheem Lateef Al-Awsi, Saade Abdalkareem Jasim, Shireen Ali Obaid,

Background: Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disorder (EBV-PTLD) is a known and serious condition for the hematopoietic stem cell transplantation. This study evaluated the prevalence of EBV in umbilical cord samples of healthy pregnant women in Baghdad, Iraq.  Methods: 800 umbilical cord blood (UCB) samples were collected from healthy pregnant women. The ...

Journal: :Arquivos brasileiros de oftalmologia 2007
Bruno F Fernandes Emilia Antecka Gilberto dos Passos Miguel N Burnier

PURPOSE The purpose of our study was to detect circulating malignant cells (CMCs) in oversea-shipped blood samples of patients with uveal melanoma diagnosed in Brazil. METHODOS: Melan-A and tyrosinase were the two markers used for the detection of CMCs, using reverse transcriptase nested polymerase chain reaction (RT-nested-PCR) in 6 patients with uveal melanoma. The expression of beta-actin an...

Journal: :Applied and environmental microbiology 1998
J Green K Henshilwood C I Gallimore D W Brown D N Lees

We describe the evaluation of a nested reverse transcriptase PCR (RT-PCR) procedure for the detection of small round-structured viruses (SRSVs) in molluscan shellfish and the application of this assay for the detection of SRSVs in commercially produced shellfish and in shellfish implicated in outbreaks of gastroenteritis. The range of virus strains detected and the sensitivity of detection were...

A Foroughi , A Mohammadi , A Salimi-Jeda , B Alirezaie , S Shahmahmoodi ,

No abstract this article: A Nested RT-PCR Method for Confirming the Absence of all Three Types of Polioviruses in Primates and Human Cells Used in Vaccine Production

Background: Bovine leukaemia virus (BLV) is the primary cause of enzootic bovine leukaemia which belongs to the HTLV/BLV group of oncogenic retroviruses including Human T-cell leukaemia virus types 1, 2, and 3 and simian T-lymphotropic virus. Due to daily consumption of milk, meat and other dairy by-products, a possible role in appearing human cancers can be assumed for BLV. Materials and Metho...

Journal: :Memorias del Instituto de Investigaciones en Ciencias de la Salud 2017

Journal: :IOSR Journal of Agriculture and Veterinary Science 2012

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