Gas chromatography-mass spectrometry-based metabolite profiling can lead to an understanding of various disease mechanisms as well as to identifying new diagnostic biomarkers by comparing the metabolites related in quantification. However, the unexpected transformation of urinary steroids during enzymatic hydrolysis with Helix pomatia could result in an underestimation or overestimation of thei...

The extracellular matrix is a poorly studied, yet important component of dental biofilms. Fluorescence lectin-binding analysis (FLBA) is a powerful tool to characterize glycoconjugates in the biofilm matrix. This study aimed to systematically investigate the ability of 75 fluorescently labeled lectins to visualize and quantify extracellular glycoconjugates in dental biofilms. Lectin binding was...

Glycoproteins of Sendai virus were successfully isolated on a column of Helix pomatia Lectin-Sepharose 6MB following solubilization with Nonidet P-40. This technique can be used as a preparative step for the study of viral glycoproteins.

Neuraminidase treatment of human peripheral blood lymphocytes uncovers cell surface receptors that bind purified A hemagglutinin from the snail Helix pomatia. No hemagglutinin was bound to untreated lymphocytes. Binding studies with (125)I-labeled hemagglutinin suggested that the number of receptors on neuraminidase-treated lymphocytes was approximately 1.10(6)/cell. The apparent association co...

The problem of the structure of complex proteins containing carbohydrates in their molecule is still a subject of discussion. The fundamental questions which have been debated during the last decades and which have been the subject of many controversies are concerned with the nature of the carbohydrate group present in the molecule of these substances. Since the discovery of mucoproteins or muc...

Thom as K nöpfel and Peter From herz Abteilung Biophysik der Universität Ulm, D-7900 Ulm-Eselsberg. Bundesrepublik Deutschland Z. Naturforsch. 42c, 986—990 (1987); received December 29, 1986/February 16. 1987 Potential Sensitive Dye, Fluorescent Probes, Styryl Dyes, Helix pomatia Using amino-styryl-pyridinium dyes as fluorescent probes we have studied the membrane potential in spheroidal neuron...

Bioactive products from snail slime of “Helix” specie have potential applications in preventing and/or treating several human diseases and in cancer diagnosis. However, the poor pharmacokinetics characteristics of these natural compounds limit their use. Nanotechnology offers promising solutions for the enhanced formulation of these molecules through the synthesis of nanosized drug del...