We covalently linked to regenerated cellulose filters a high-affinity monoclonal IgM produced against epitopes that reside on hepatitis B viral surface antigen (HBsAg). Conditions were established whereby as much as 250 micrograms of anti-HBsAg IgM could be linked to 2-4 mg of regenerated cellulose acetate by using cyanogen bromide and trichloro-s-triazine coupling agents. The immunoreactivity ...

The study aimed to determine the association of additional N-glycosylation mutations in the major hydrophilic region (MHR) of hepatitis B virus (HBV) S gene with hepatocellular carcinoma (HCC) occurrence in HBsAg/anti-HBs coexistent patients. A total of 288 HBsAg/anti-HBs coexistent patients and 490 single HBsAg-positive patients were enrolled, including 193 with HCC, 433 with chronic hepatitis...

this work examined the simultaneous saccharification and fermentation (ssf) process for the biological conversion rice wastewater into ethanol using co-culture of aspergillus niger (a. niger) and saccharomyces cerevisiae (s. cerevisiae) in batch condition. in this study, the a. niger and s. cerevisiae were used for hydrolysis and production of ethanol from rice wastewater, respectively. the effe...

Hepatitis B virus (HBV) and human immunodeficiency virus (HIV) are hyperendemic in sub-Saharan Africa. The HBV genotypes prevailing in HIV-infected Africans are unknown. Our aim was to determine the HBV genotypes in HIV-infected participants and to identify clinically significant HBV mutations. From 71 HBV DNA(+ve) HIV-infected participants, 49 basic core promoter/precore (BCP/PC) and 29 comple...

using probiotics can control pathogens by a variety of mechanisms. probiotics can promote growth performance and have, therefore, become increasingly important in the aquaculture industry. convict cichlid belongs to the family of cichlidae and is known for its rapid development in laboratory conditions and is suitable for behavioral examinations. the aim of this study was to evaluate the effect...

We investigated the effects of hepatitis B virus (HBV) S/C double gene loci antisense locked nucleic acid on replication and expression of HBV in hepatitis transgenic mice. HBV mice (N = 30) were randomly divided into five groups of six mice: 5% glucose solution control, empty liposome control, single-target S, single-target C, and dual-target SC groups. An antisense locked nucleic acid fragmen...

HBs protein is encoded by the pre-S and S genome, and the detection of HBs antigen (HBsAg) in sera is an initial step for the diagnosis of hepatitis B virus (HBV) infection. The “a” determinant region (amino acid residues 124–147) is the main epitope for inducing a protective immune response. Mutations in the “a” determinant region, known as escape mutants, affect antigenicity and hinder attemp...

pomegranate fruit is a rich source of bioactive compounds. the serious concern over unprocessed fruit juices is microbial contamination, which effectively inactivated by thermal processing, but it significantly affects juice functional compounds. therefore, the effect of gamma irradiation and ultrasonic on inoculated microbial to pomegranate juices was studied.two pomegranate cultivars were pur...