The present study was carried out to study the effect of inovo ochratoxin A (OTA) injection in Highline layer eggs on calcium metabolism and blood biochemical parameters of embryos and after the hatch. At day 10 of embryonic development, one hundred and sixty-two fertile eggs were individually weighed and divided into two equal treatments. The first treatment (control) consisted of the individu...

Bovine follicular oocytes matured in vitro were fertilized in vitro using epididymal spermatozoa from five different bulls and then cultured to the blastocyst stage in vitro. The fertilization rate, based on one pair of pronuclei and presence of one sperm tail, ranged from 55.2 to 64.3%. Embryo development (cleavage to blastocyst stage) ranged from 21.4 to 31.0% of the cultured ova reaching 8 c...

It has been repeatedly demonstrated that estrous expression before fixed-time AI (TAI) results in increased pregnancy success. Therefore, the objective of this experiment was to determine if preblastocyst embryonic developmental characteristics differed from heifers that did or did not exhibit estrus before TAI. Beef heifers (n = 113) were synchronized using the 5-d CO-Synch + controlled intern...

background: progress achieved in culture media formulations have resulted led to an improvement in maintaining the mammalian embryo in culture throughout the preimplantation and pre-attachment period. objective: the objective of this study was to evaluate the effect of various concentrations of myo-inositol during in vitro fertilization of bovine oocytes on subsequent embryo development. materi...

Rainbow trout embryos were chronically exposed to silver (as AgNO3) in moderately hard water (120 mg CaCO3/L, 0.70 mM Cl-, 1.3 mg/L dissolved organic matter. 12.3+/-0.1 degrees C) at nominal concentrations of 0.1, 1, and 10 microg/L (measured = 0.117+/-0.008, 1.22+/-0.16, and 13.51+/-1.58 microg/L, respectively) to investigate the effects on mortality, ionoregulation, and silver uptake and dist...

This study aims to determine the effect of in-ovo injection L-arginine into local chicken eggs on embryo development and chick performance chicken. A total 160 were incubated using semi-automatic incubator with temperature 37-38C relative humidity 55-65%. The injected solution has a concentration 0.5% (m/v). In treatment was divided 4 groups, first without (control), second 0.2 mL (m/v)/egg, th...

We studied the artificial incubation method of red swamp crayfish Procambarus clarkii embryos using cell culture plates. In first experiment, cleavage stage or egg-nauplius were individually cultured at 20°C in well plates with different water volumes per [12 (4 ml), 24 (2 48 (1 ml) and 96 (0.25 ml)]. second 12-well 15, 20 25°C. Almost all died without hatching. Egg-nauplius survived to hatch. ...

BACKGROUND The present study was undertaken to examine the usefulness of both vitrification and assisted hatching (AH) on blastocysts that originate from embryos showing different qualities during their cleavage stage. METHODS A total of 281 blastocysts were vitrified (93 vitrification-warming cycles) in a mixture of ethylene glycol-dimethylsulphoxide-Ficoll and sucrose using the Hemi-Straw (...

Advice is provided on five questions that were addressed by subjecting emu eggs, as a model for kiwi eggs, to transport-induced jarring during three stages of embryo development. The jarring associated with two kiwi egg recovery operations was also measured. Jarring during transportation had no demonstrable effect on embryo development, the need for hatching assistance, or the incidence of embr...

Recent studies found fish egg-specific bacterial communities that changed over the course of embryogenesis, suggesting an interaction between the developing host and its microbiota. Indeed, single-strain infections demonstrated that the virulence of opportunistic bacteria is influenced by environmental factors and host immune genes. However, the interplay between a fish embryo host and its micr...