Precise control of chromosome pairing is vital for conferring meiotic, and hence reproductive, stability in sexually reproducing polyploids. Apart from the Ph1 locus of wheat that suppresses homeologous pairing, little is known about the activity of genes that contribute to the cytological diploidization of allopolyploids. In oilseed rape (Brassica napus) haploids, the amount of chromosome pair...

Cultivated and wild potato species synthesize a wide variety of steroidal glycoalkaloids (GA) that may affect either human health or biotic stress resistance. Therefore, GA composition must be a major criterion in the evaluation of breeding products when species genomes are merged and/or manipulated. This work reports the results of GA analysis performed on unique haploid (2n=2x=24) plants obta...

Resynthesized (RS) oilseed rape (Brassica napus L.) is potentially of great interest for hybrid breeding. However, a major problem with the direct use of RS B. napus is the quality of seed oil (high level of erucic acid) and seed meal (high glucosinolate content), which does not comply with double-low quality oilseed rape. Thus, additional developments are needed before RS B. napus can be intro...

Two haploid cell lines have been established from androgenetic embryos of the frog, Rana pipiens; one line has been maintained in culture for 150 generations, the other for 200 generations. Karyotypes of the two lines agree well with the standard for the species although some chromosomes show small differences in length. The cells multiply in the same defined basal medium used for culture of ot...

The seedling traits is a simple and non-destructive methodology used to identify haploids/diploids in maize. This study aimed at establishing an optimal germination temperature evaluate this verify its effectiveness. Haploid diploid seeds, obtained from crosses with the haploidy inducer TAIL9, were classified using R1-Navajo marker placed germinated growth chambers maintained different temperat...

The aim of the study was to development rice microspores in anther culture vitro , structure androclinic callus identify cyto-embryological features formation morphogenic structures culture, obtain doubled haploids ( Oryza sativa L .) and accelerate valuable breeding material with desired properties. Within framework this study, results a cyto-histological haploid androgenesis were obtained, wh...

Haploids are a valuable tool for genomic studies in higher plants, especially those with huge genome size and long juvenile periods, such as conifers. In these species, megagametophyte cultures have been widely used to obtain haploid callus and somatic embryogenic lines. One of the main problems associated with tissue culture is the potential genetic instability of the regenerants. Because of t...