Total and differential haemocyte counts following repeated haemolymph withdrawals were made in V instar larvae of P. demoleus. While total count showed a steady reduction reaching its lowest in prepupal stage, much variation was observed in the relative percentage of various cell types. Further, the repeated removals of haemolymph from V instar larvae and pupae affected imaginal moulting and le...

A larval moult in the tobacco hornworm, Manduca sexta, involves an endocrine cascade that begins with the release of a cerebral peptide hormone, the prothoracicotropic hormone (PTTH). The release of PTTH is gated, occurs during the scotophase and appears to be developmentally cued. In fourth instar Manduca larvae, PTTH release into the haemolymph occurs as a single burst over a few hours during...

In fourth instar larvae of Camptochironomus tentans, net sodium uptake from 2 mM-NaCl has an electrogenic component. During net uptake the transepithelial potential (TEP) alters from a value of approximately - 40 mV (sign refers to haemolymph), in depleted animals, to approximately o mV. The TEP in depleted larvae is dependent upon external sodium concentration above about I mM-Na+, becoming in...

Larvae of Culiseta inornata (Williston) can survive and complete development in dilutions of sea water ranging from 50-700 mosmol kg-1. The larvae hyperregulate with regard to haemolymph osmotic concentration in dilute media (50-400 mosmol kg-1) and osmoconform when external salinities exceed 400 mosmol kg-1. This pattern of osmoregulation is distinct from that observed in freshwater and saline...

Insect Haemolymph, Hormone Binding Proteins, Juvenile Hormone, Photoaffinity Labelling, Manduca sexta, Locusta migratoria Juvenile hormone III, i.e. methyl 10,1 l-epoxy-3,7-dimethyl-2£’,6£'-dodecadienoate, was used for photoaffinity labelling of JH-binding proteins. Irradiation of haemolymph from Manduca sexta and Locusta migratoria in the presence of racemic [10-3H]JH-III results in covalent i...

Different physico-chemical properties of the haemolymph of various chilopod (Lithobius forficatus and Scolopendra cingulata) and diplopod species (Rhapidostreptus virgator, Chicobolus spec.) were investigated. Haemolypmph pH ranged from 7.0 to 8.5 being slightly more alkaline in the millipede species. Protein content and osmolarity was higher in chilopods (between 296 and 371 mOsm and 65 to 73 ...

in this research, white spot syndrome virus (wssv) with 105.4ld50.ml-1 inactivated with gamma irradiation at a dose rate of 14-15 kgy.sec-1 was used as a vaccine. three hundred and sixty shrimp of litopenaeus vannamei, medium body weight (mbw) 1.0±0.17g), were prepared and experimentally exposed. they were distributed among four groups, inclusing vaccinated-virus exposed (t1), vaccinated-nonvir...

A lectin detected in haemolymph from the Australian spiny lobster Panulirus cygnus agglutinated human ABO Group A cells to a higher titre than Group O or B. The lectin also agglutinated rat and sheep erythrocytes, with reactivity with rat erythrocytes strongly enhanced by treatment with the proteolytic enzyme papain, an observation consistent with reactivity via a glycolipid. The lectin, purifi...

The haemolymph total protein concentration and protein profile of C madrasensis exposed to different salinities were studied. The animals were maintained at salinities 6 ,12,24 and 36 ppt for one month. The serum total protein concentration showed significant reduction at 6 and 36 ppt. The haemolymph protein profile obtained by SDS-PAGE also varied with difference in salinities. There were four...

Contact-rehydration in terrestrial slugs involves a specific drinking behaviour during which water is rapidly absorbed through the integument of the foot. When dehydrated slugs were placed on wet filter paper containing 14C-inulin, they displayed the characteristic drinking posture and absorbed both water and 14C-inulin. Samples of haemolymph from dehydrated slugs after 12 min of contact-rehydr...