Vacuolar H(+)-ATPases are ubiquitous multisubunit complexes mediating the ATP-dependent transport of protons. In addition to their role in acidifying the lumen of various intracellular organelles, vacuolar H(+)-ATPases fulfill special tasks in the kidney. Vacuolar H(+)-ATPases are expressed in the plasma membrane in the kidney almost along the entire length of the nephron with apical and/or bas...

In the gills of freshwater teleost fishes, vacuolar proton-ATPase (V-H(+)-ATPase) is found on the apical membrane of pavement and chloride (Na(+)/K(+)-ATPase-rich) cells, and is an important transporter for energizing Na(+) uptake and H(+) excretion. In the gills of elasmobranch fishes, the V-H(+)-ATPase has not been extensively studied and its expression in freshwater individuals has not been ...

introduction: the regulation of the body fluid content (osmoregulation) of an aquatic animal, is performed by several organs. in fish, osmoregulatory mechanisms are based on the function of specialized cells (ionocytes) located in various tissues and organs including gills. na+, k+-atpase is one of the main osmoregulatory enzymes enabling the use of atp as a source of energy for ion transport t...

The structure, ultrastructure and osmoregulatory function of the branchial chamber were investigated in Palaemon elegans (Rathke, 1837) by light microscope, electron microscope and immunohistochemistry methods, respectively. Each chamber possesses a branchiostegite, 3 epipodites and 8 phyllobranchiate gills. The lamellae are attached along the two outer surfaces of the triangular gill axis. Ult...

The plant plasma membrane H(+)-ATPase is activated by the binding of 14-3-3 protein to the C-terminal region of the enzyme, thus forming an H(+)-ATPase-14-3-3 complex that can be stabilized by the fungal toxin fusicoccin. A novel 14-3-3 binding motif, QQXYpT(948)V, at the C terminus of the H(+)-ATPase is identified and characterized, and the protein kinase activity in the plasma membrane fracti...