objective: β-thalassemia is caused by absence or reduction of β-globin chain synthesis. one of the effective therapeutic methods for this disease can be gene therapy by viral vectors. the capacity of lentiviral vectors is approximately 8 kb, we designed a 6 kb construct containing mini lcr and β-globin gene instead of lcr region. the aim of this study is to make a recombinant lentiviruses conta...

Cystic fibrosis (CF) is the result of mutations in the cystic fibrosis transmembrane conductance regulator (CFTR). CF-related diabetes affects 50% of adult CF patients. How CFTR deficiency predisposes to diabetes is unknown. Herein, we examined the impact of the most frequent cftr mutation in humans, deletion of phenylalanine at position 508 (ΔF508), on glucose homeostasis in mice. We compared ...

Liver receptor homolog-1 (LRH-1) is an orphan nuclear receptor that belongs to the NR5A subgroup of nuclear receptors. LRH-1 induces key genes to regulate metabolic process, ovarian function, cancer cell proliferation, and steroidogenesis. In the breast, LRH-1 modulates and synergizes with endogenous estrogen signaling to promote breast cancer cell proliferation. We used small interfering RNA k...

PURPOSE To repair the segmental orbital rim defects of dogs with three-dimensional (3D) tissue-engineered constructs derived from culturing autogenous bone marrow stromal cells (BMSCs) on β-tricalcium phosphate (β-TCP) scaffolds. METHODS A 25-mm segmental defect on the canine inferior orbital rim was created. BMSCs were isolated and osteogenically induced in vitro, then were seeded onto 3D β-...

The gross morphological features of the eggs and eggshells (chorions) of two Lepidoptera species, Pericallia ricini and Ariadne merione were revealed for the first time by scanning and transmission electron microscopy. These two insect pests are extremely serious threats for many crops, mainly in India, but also in several other regions of the world. Micro-Raman and ATR FT-IR spectroscopy were ...

The dynamics and interaction of quantized vortices in Bose-Einstein condensates (BECs) are investigated by using the two-dimensional Gross-Pitaevskii equation (GPE) with/without an angular momentum rotation term. If all vortices have the same winding number, they would rotate around the trap center but never collide. In contrast, if the winding numbers are different, their interaction highly de...

The anticataract activity of Acorus calamus roots extract against induced hydrogen peroxide cataractogenesis was assessed in Goat lenses. The in vitro phase of study was performed on lenses from Goat eyes incubated for 52 h at 37oC in Dulbecco Modified Eagle Medium (DMEM) containing H2O2 (0.5 mM) (Group I), in DMEM containing H2O2 (0.5 mM) and methanolic extract of Acorus calamus (MEAC), (Group...