A novel ligand fishing assay was established to screen triplex DNA binders from complicated samples by a combination of immobilization of triplex DNA on agarose beads and high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). The biotinylated oligodeoxynucleotides were first bound to the streptavidin agarose beads and then incubated with the duplex DNA a...

A study was conducted with an objective to compare the efficacy of bentonite (BT), Spirulina platensis (SP) and glucomannan mycotoxin binders (GMA) on aflatoxicosis in broiler breeders. Three levels of AF, three binders and combination of different levels of AF with binders were evaluated. The AF fed at the levels of 300, 400 and 500ppb for three periods, each with duration of three weeks in br...

BACKGROUND Vascular calcification is a major contributing factor to mortality in end stage renal disease (ESRD). Despite the efficacy of phosphate binders to improve hyperphosphatemia, data on vascular calcification are less clear. There seems to be a difference in attenuation or delay in progression between different binders. In this in vitro experiment we tested whether phosphate binders coul...

Material extrusion additive manufacturing of metal (metal MEX), which is one the 3D printing processes, has gained more interests because its simplicity and economics. Metal MEX process similar to conventional injection moulding (MIM) process, consisting feedstock preparation powder polymer binders, layer-by-layer MEX) or create green parts, debinding remove binders sintering consolidated metal...

Lon W. Whitlow Department of Animal Science North Carolina State University Raleigh, NC 27695 Phone: 919-515-7602 Fax: 919-515-2152 Email: [email protected] Summary Considerable research has been directed at finding methods to prevent toxicity of mycotoxins. Some of the approaches have included mycotoxin separation from contaminated feeds, detoxification and inactivation. Detoxification and ...

Peptide binding to HLA molecules is a critical step in induction and regulation of T-cell mediated immune responses. Because of combinatorial complexity of immune responses, systematic studies require combination of computational methods and experimentation. Most of available computational predictions are based on discriminating binders from non-binders based on use of suitable prediction thres...

B. Grabowska, M. Holtzer, R. Dańko, M. Górny, A. Bobrowski, E. Olejnik; AGH University of Science and Technology, Faculty of Foundry Engineering, Krakow, Poland Possibilities of cross-linking of new polymer binders from the BioCo group, their hardening in moulding sands at the application of cross-linking agents both physical and chemical are presented. Their thermal stability was determined. I...

Short peptides can be designed in silico and synthesized through automated techniques, making them advantageous and versatile protein binders. A number of docking-based algorithms allow for a computational screening of peptides as binders. Here we developed ex-novo peptides targeting the maltose site of the Maltose Binding Protein, the prototypical system for the study of protein ligand recogni...

A study was conducted with an objective to compare the efficacy of bentonite (BT), Spirulina platensis (SP) and glucomannan mycotoxin binders (GMA) on aflatoxicosis in broiler breeders. Three levels of AF, three binders and combination of different levels of AF with binders were evaluated. The Aflatoxin B1 (AF) fed at the levels of 300, 400 and 500ppb for three periods, each with duration of th...

A study was conducted with an objective to compare the efficacy of bentonite (BT), Spirulina platensis (SP) and glucomannan mycotoxin binders (GMA) on aflatoxicosis in broiler breeders. Three levels of AF, three binders and combination of different levels of AF with binders were evaluated. The AF fed at the levels of 300, 400 and 500ppb for three periods, each with duration of three weeks in br...