Human vascular cells are capable of stimudium obtained from cord veins was found lating granulopoiesis in agar culture of to be highly active in comparison to that human bone marrow cells. This effect was obtained from peripheral leukocytes. Enobtained by including vein fragments in dothelial cells within the bone marrow the culture or by using endothelial cells cavity are suggested as a local ...

BACKGROUND The role of flow cytometry (FCM) in diagnosing myelodysplastic syndromes (MDS) remains controversial, because analysis of myeloid maturation may involve subjective interpretation of sometimes subtle patterns on multiparameter FCM. METHODS Using six-parameter marker combinations known to be useful in evaluating the myeloid compartment in MDS, we measured objective immunophenotypic d...

Megakaryocytopoiesis and granulopoiesis of marrow cells from W/Wv mice were studied using a continuous liquid marrow culture system. Cells in the suspension phase were assayed weekly over a 16-week period for total nucleated cells, megakaryocytes, granulocytes, megakaryocytes and granulocyte-macrophage progenitor cells (CFU-Ms, CFU-GMs), and spleen colony-forming cells (CFU-Ss). Without hydroco...

BACKGROUND MicroRNAs (miRNAs) are coordinators of cellular differentiation, including granulopoiesis. Although differential expression of many miRNAs is associated with the maturation of granulocytes, analysis of differentially expressed miRNAs and their cellular localization across all stages of granulopoiesis, starting from hemopoietic stems cells, is not well characterized. METHODS We anal...

Evaluation of proliferative activity of granulocytic progenitor cells from marrows of neutropenic patients was performed by utilizing in vitro culture methods measuring granulocytic colony-forming capacity (CFC) and the proportion of granulocytic progenitor cells in the DNA synthetic (S) phase of the cell cycle. Except for low CFC values found in five patients with myeloid hypoplasia, the remai...