2. Copley CG, Egglestone SI. Auxotyping of Neisseria gonorrhoeae isolated in the United Kingdom. J Med Microbiol. 1983;16:295–302. 3. Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979;7:1513–23. DOI: 10.1093/ nar/7.6.1513 4. Papadimos TJ, Escamilla J, Batchelor RA, Lane EW, Biddle JW. Antimicrobial susceptibility of Neisser...

The severe adverse effects of gonococcal infection on human fertility suggests that Neisseria gonorrhoeae would exert powerful selection for the development of a protective immune response in humans. N. gonorrhoeae is an obligate human pathogen and must persist in humans to survive. Since it is an ecologically successful organism, it must have evolved strategies to evade any human immune respon...

The development of effective prophylactic agents against gonorrhea and the study of adaptation by Neisseria gonorrhoeae to the urogenital mucosa are hindered by the lack of a well-established animal model of gonococcal genital tract infection. Here, a murine model of long-term gonococcal genital tract infection is described. Female BALB/c mice were treated with 17-beta-estradiol and inoculated ...

In men with gonococcal urethritis, the urethral epithelial cell is a site of infection. To study the pathogenesis of gonorrhea in this cell type, we have developed a method to culture primary human urethral epithelial cells obtained at the time of urologic surgery. Fluorescent analysis demonstrated that 100% of the cells stained for keratin. Microscopic analyses indicated that these epithelial ...

In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase (LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo induced a significant increase (~35-fold) in mRNA transcripts of the inducible isoform of NOS. Parado...

Non-Gonococcal Urethritis.-All inflammatory processes of the male urethra presumed due to sexual intercourse, which were not caused by N. gonorrhoea, H. ducreyi, T. pallidum, the tubercle bacillus, protozoan, metazoan organisms, fungi, mechanical irritants, or tumours, were classified as non-gonococcal urethritis. Acute Urethritis, Non-Gonococcal.-Non-gonococcal urethral infections in which the...

Gonococcal infection in the postantibiotic era continues to cause disseminated and severe disease in some patients. The differential diagnosis of pain in the lower abdomen in young women is difficult. Our case report described a 19-year-old patient who presented with acute abdomen as a result of Gonococcal infection, assessed as a local complication, pelveoperitonitis: pelvic inflammatory disea...

Neisseria gonorrhoeae is one of the most prevalent sexually transmitted infections worldwide. This obligate human pathogen has been extensively studied in vitro, where bacterial factors that are known to contribute to gonococcal disease and their regulation are relatively well defined. However, these in vitro experimental conditions only loosely replicate the host specific environment encounter...

OBJECTIVE To determine the association of Mycoplasma genitalium and Ureaplasma urealyticum in symptomatic male patients presenting with non-gonococcal urethritis in a sexually transmitted infection clinic in Hong Kong. DESIGN Cross-sectional study. SETTING A sexually transmitted infection clinic, Department of Health, Centre for Health Protection, Hong Kong. PATIENTS A cohort of consecuti...