Synaptic vesicle exocytosis is triggered by rises in calcium up to 100 microM at the site of vesicle fusion. The synaptic vesicle proteins synaptotagmin 1 and 2 (Syt I and Syt II) bind calcium at similarly high concentrations and have been proposed as the calcium sensors for fast neurotransmitter release. However, 1 microM calcium produces tonic transmitter release at photoreceptor and bipolar ...

Goldfish have been used for cold acclimation studies, which have focused on changes in glycolytic and oxidative enzymes or alterations in lipid composition in skeletal muscle. Here we examine the effects of cold acclimation on the functional properties of isolated mitochondria and permeabilized fibers from goldfish white skeletal muscle, focusing on understanding the types of changes that occur...

The present study evaluated the effects of both intraperitoneal (i.p.) and intracerebroventricular administration of selective Y1 [(Leu , Pro)-NPY] and Y2 [(Pro , Tyr)-NPY (13–36)] receptor agonists on food intake in satiated goldfish. Food intake (FI) was significantly increased by central administration of the Y1 agonist (1 mg), but not by the Y2 agonist, at 2 h postinjection. The feeding inc...

 Environmental enrichment is one of the methods for improving fish growth performance. In the present trial, the effects of both light color and music on growth performance and survival of goldfish (Carassius auratus) (initial mean weight: 4.15±0.08 g) were investigated for 2 months. Two light color treatments (red light and white light) and three music treatments (M0: without music, M30:30 min...

Progestin induction of oocyte maturation (OM) in fish is a useful model for investigating endocrine disruption of nongenomic steroid actions. Although diethylstilbestrol (DES) analogs have been shown to mimic the actions of progestins to induce meiotic maturation of goldfish and zebrafish oocytes, their molecular mechanisms of action remain unclear. The ability of these endocrine-disrupting che...

Jiang Q, He M, Ko WK, Wong AO. Kisspeptin induction of somatolactinrelease in goldfish pituitary cells: functional role of cAMP/PKA-, PLC/PKC-, and Ca /calmodulin-dependent cascades. Am J Physiol Endocrinol Metab 307: E872–E884, 2014. First published September 2, 2014; doi:10.1152/ajpendo.00321.2014.— Although the importance of kisspeptin in the pituitary is firmly established, the signaling me...

In mammals, growth hormone-releasing hormone (GHRH) is the most important neuroendocrine factor that stimulates the release of growth hormone (GH) from the anterior pituitary. In nonmammalian vertebrates, however, the previously named GHRH-like peptides were unable to demonstrate robust GH-releasing activities. In this article, we provide evidence that these GHRH-like peptides are homologues of...

In April and June 2014, mortalities of goldfish occurred at Korea. The principal signs included pale gills, severely enlarged softened spleen kidney red liver. Moreover, the histopathological characteristics observed mainly in hematopoietic tissues kidney, gill lamellae intestine. predominant changes were severe necrosis tissue. addition, nucleus exhibiting peripherally displaced chromatin part...