Specific IgE antibody levels in the serum of patients with proven gnathostomiasis and in those with intermittent cutaneous migratory swelling (CMS) were determined by the enzyme-linked immunosorbent assay (ELISA) using somatic extract and excretory-secretory (ES) products of Gnathostoma spinigerum infective larvae as antigens. The third stage larval used were obtained from naturally infected ee...

Macroscopic, description.?The specimen consisted of an irregular reddish-yellow firm tumour and a living round worm. The tumour measured '?0 X 6.5 X 3.0 cm. and was situated in the great omentum below the greater curvature oi Mie stomach. It was apparently covered with smooth dull-looking peritoneum and at one place was overlaid by a few dark-red blood ('lots. On serial section it was found to ...

An endoparasite survey of a small pride of African lions (Panthera leo) was conducted at Intu Afrika Kalahari Game Reserve, southwestern Namibia, during winter and summer of 2003 and 2004, respectively. Overall, 23 fresh lion scats were collected opportunistically during fieldwork trials. A flotation technique was employed for the diagnosis of parasites. Three nematodes, Ancylostoma braziliense...

Gnathostoma are parasites of wild mammals and larval stages of 3 native species, G. spinigerum, G. doloresi and G. nipponicum have been found in cold blooded vertebrates. Moreover, larval G. hispidum has been found in loaches imported to Japan from east Asia (Akahane et ai, 1982). Among these species, G. spinigerum had been considered to be the only causative agent of gnathostomiasis until the ...

Eosinophilic meningitis is defined by the presence of 10 or more eosinophils per microliter in the cerebrospinal fluid (CSF) or eosinophilia of at least 10% of the total CSF leucocyte count.1 It is rare in Western countries, where it is sometimes seen in association with tuberculosis, syphilis, and coccidioidomycosis.2 Worldwide, the most common cause of eosinophilic meningitis is invasion of t...

Typically, spinal gnathostomiasis is presented as radiculomyelitis. Here, we report a spinal epidural hematoma caused by Gnathostoma spinigerum. A 28-year-old man presented with acute chest pain for 1 hour. The pain was very severe, with dull aching on his left chest and toward his back. At the hospital, he developed paraplegia and sensory loss up to the T4 level. Physical examination revealed ...

Immunohistochemical localization of antigens in advanced third-stage larvae of Gnathostoma spinigerum (GsAL3) was studied by indirect enzyme immunostaining using 7 G. spinigerum specific monoclonal antibodies, FS-3D11, SS-5H5, SK-6C4, SK-4E1, SK-7G6, SD-8D4 and SA-9B5. All these MAb belong to the IgG1 subclass and only FS-3D11 and SS-5H5 recognize carbohydrate determinants. Each MAb exhibited a...

To investigate the distribution of Gnathostoma spp in Ho Chi Minh City (HCM city), 1,081 eels were purchased from a local market twice a month from March 1998 to February 1999. Infective larvae of Gnathostoma spp detected from the flesh and liver of eels by the press preparation technique were examined and identified. Three hundred and fifty advanced third-stage larvae were recovered from liver...

Gnathostomiasis caused by Gnathostoma spinigerum, is a hazardous food- borne helminthic zoonosis, and is endemic especially in developing countries in Asia. Definitive diagnosis, relying upon identification of worms from human tissues or body, is rarely accomplished. Consequently, sensitive supporting tools such as serological tests have been used widely. But these methods are time con- suming,...