13C nmr spectral parameters were measured for intact bovine nasal cartilage tissue, the purified proteoglycan aggregate, and chondroitin 4-sulfate. A comparison of integrated intensities obtained for four different samples of fresh tissue with an ethylene glycol standard indicated that at least 80% of the total glycosaminoglycan carbons in the tissue contributed to the spectrum. This result was...

The interaction of monolayer normal human or normal rat cells with suspension Walker rat tumor cells was demonstrated cytologically, during a cocultivation period of thirty days. At ten days, Walker rat tumor cells were interiorized in the cytoplasm of the normal monolayer host cells. At twenty days, degeneration of the interiorized tumor cells followed by mucification led to glycosaminoglycan-...

Our previous report has shown that the shapes of calcium oxalate crystals formed in a supersaturated solution with glycosaminoglycan varied with glycosaminoglycan species. The present study was conducted to demonstrate the effect of magnesium on the shapes of calcium oxalate crystals with glycosaminoglycan by optical and scanning electron microscopy. In the presence of magnesium, the phenomenon...

PURPOSE The present study was undertaken to examine the relationship between choroidal permeability and scleral glycosaminoglycan synthesis rates during the development of and recovery from form deprivation myopia. METHODS Form deprivation myopia was induced in chicks for 10 days and was followed by a period of unrestricted vision for 0 to 15 days (recovery). Choroidal permeability was quanti...

Brefeldin A has dramatic, well-documented, effects on the structural and functional organization of the Golgi complex. We have examined the effects of brefeldin A (BFA) on the Golgi-localized synthesis and addition of chondroitin sulfate glycosaminoglycan carbohydrate side chains. BFA caused a dose-dependent inhibition of chondroitin sulfate glycosaminoglycan elongation and sulfation onto the c...

There was no difference in the incidence of recurrence of superficial digital flexor tendinitis in horses treated conservatively compared with those treated by intralesional sodium hyaluronate, glycosaminoglycan polysulfate, or systemic glycosaminoglycan polysulfate. Author’s address: Centre for Equine Studies, Animal Health Trust, Snailwell Rd., Newmarket, Suffolk CB8 8JH, England. r 1997 AAEP.

Glycosaminoglycan synthesis and secretion by primate arterial smooth muscle have been examined in cell culture. Mass cultures of diploid primate arterial smooth muscle cells were either double labeled with [35S]sulfate and [3H]acetate or single labeled with [3H]glucosamine for 24 h and glycosaminoglycans were extracted and isolated from the culture medium. Incorporation of labeled precursors in...