A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of t...

Multiple herbicide-resistant waterhemp ( Amaranthus tuberculatus (Moq.) J.D. Sauer) has limited herbicide options for Iowa row crop production. Soybean Glycine max (L.) Merr.) cultivars and corn Zea mays L.) hybrids resistant to 2,4-D, dicamba, glufosinate have provided more management. A probability-based survey of fields was conducted in fall 2019 wherein seeds were collected their susceptibi...

Changes in seeds of soybeans (Glycine max [L.] Merr. var. Wayne) which occur during accelerated aging (41 C, 100% relative humidity) showed subsequent loss of vigor, a decline in early respiratory activity, increased leakage of electrolytes, losses of as much as 10% dry weight from imbibing cotyledons, and a decrease in the swelling response of the imbibing system (seed plus H(2)O). Each of the...

In the soybean (Glycine max (L.) Merr.), the gene combination Fg1 Fg3 is responsible for the glycosylation in the biosynthesis of kaempferol triglucoside (K9) in leaves. The presence of K9 is associated with reduction in chlorophyll content, specific leaf mass, photosynthetic rate and stomatal frequency. Blocking the action of Fg1 Fg3 with the magenta flower gene wm prevents formation of K9 and...

Characterization of nodule growth and functioning, phosphorus status of plant tissues and hostplant growth of nodulated soybean (Glycine max L. Merr.) plants grown under different phosphorus conditions was studied in order to evaluate the role of phosphorus in symbiotic nitrogen fixation. Phosphorus deficiency treatment decreased the whole plant fresh and dry mass, nodule weight, number and fun...

Salinity stress restricts growth of soybean plant [Glycine max (L.) Merr.]. Glycine betaine (GB) is among osmoprotectant compounds that are produced in tolerant plant species in response to environmental stresses. Soybean is sensitive to soil salinity and is classified as a low-ccumulator of glycine betaine. Therefore, this study was performed to evaluate the salinity tolerance of two contrasti...