The stability of peptide DNA condensates was examined after introducing glutaraldehyde to cross-link surface amine groups. A 20 amino acid peptide (CWK(18)) was used to condense DNA into small (70 nm) condensates. The reaction between glutaraldehyde and peptide DNA condensates was indirectly monitored using a fluorescence-based assay to establish reaction completion in 4-5 h when using glutaral...

Electron micrographs (EMs) of central nervous tissue perfused with glutaraldehyde and post-fixed with osmium tetroxide exhibit more extracellular space in superficial than in deep regions of the brain. It was postulated that this is due to the availability to the surface tissue of the cerebrospinal fluid in the subarachnoidal space. It was assumed that in addition to the extracellular material ...

Introduction: Orthodontist must take precautionary measures to protect patients from cross-infection by contaminated materials received the manufacturers. To minimize incidence of prior bonding an invitro study was done assess antibacterial activity chlorhexidine, povidone iodine and glutaraldehyde. Aim: compare 2% 5% glutaraldehyde on orthodontic bonding. Materials methods: A total 36 samples ...

BACKGROUND Glutaraldehyde is the best disinfectant for fibreoptic endoscopes. It is also used in the processing of x ray films. A number of studies have reported eye, nose, and respiratory symptoms in exposed workers. Three individual case reports of occupational asthma in endoscopy workers and a radiographer have also been published. We describe a further seven cases of occupational asthma due...

The interacting effects of time of exposure and concentration as factors in cytotoxicity were compared for glutaraldehyde and formaldehyde. Cells from a human fibroblast cell line (WI-38) grown to confluence in 24-well trays were exposed to a range of concentrations of each agent, for periods of 4 to 24 hr. Cytotoxicity was measured by its effects on mitochondrial dehydrogenase activity, as ass...

Aqueous solutions of alkaline glutaraldehyde (buffered at pH 8.5) inactivated a standard suspension of Mycobacterium tuberculosis H37Rv faster than the corresponding acid (pH 3.7 preparation. Quantitative differences in the rate of inactivation of eight other species of Mycobacterium were determined using a 1% solution of alkaline glutaraldehyde and inactivation of residual glutaraldehyde with ...

The effect of glutaraldehyde, a commercial biocide widely used in paper and pulp industry, on the performance of laboratory-scale rotating biological contactors (RBCs) as well as biocide efficacy was studied. Biofilms were established on the RBCs and then exposed to 0 180 ppm glutaraldehyde at a dilution rate of 1.60 h. The results showed that the biofilms became acclimated to glutaraldehyde an...

We have evaluated the quality of ultrastructural preservation of erythrocyte ghosts achieved under various electron microscopic preparative conditions. Initially, negative staining was used to monitor gross morphology of the ghosts. Of several negative stains used (phosphotungstate, silicotungstate, ammonium molybdate, and uranyl acetate), all but uranyl acetate resulted in fragmentation of mem...