Human acid beta-glucosidase (glucosylceramidase; EC 3.2.1.45) cleaves the glycosidic bonds of glucosyl ceramide and synthetic beta-glucosides. Conduritol B epoxide (CBE) and its brominated derivative are mechanism-based inhibitors which bind covalently to the catalytic site of acid beta-glucosidase. Procedures using brominetritiated CBE and monospecific anti-human placental acid beta-glucosidas...

BACKGROUND In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to biofuel via fermentation. The crucial step in the production of biofuel from biomass is the enzymatic saccharification. Many of the commercial cellulase enzyme cocktails, such as Spezyme(®) CP (Genencor), Acellerase™ 1000 (Genencor), and Cellucla...

the snail caucasotachea lencoranea mousson is a pest of citrus in the north provinces of iran. in order to adopt a better control of the pest and develop optimal management strategies, an understanding of the digestive enzymes’ function is inevitable. snails were collected from some citrus orchards in the northern provinces of the country and digestive carbohydrases including ?-glucosidase, ?-g...

The risk of osteoporosis or osteopenia is known to increase after childhood cancer treatment. The purpose of this study was to evaluate patterns of bone mineral density (BMD) and to identify factors related to the decreased BMD in childhood cancer survivors. We studied 78 patients (34 boys, 44 girls) treated for childhood cancer. Twenty (25.7%) patients had lumbar BMD (LBMD) standard deviation ...

An extracellular β-glucosidase was purified from a culture filtrate of the fungus Cladosporium resinae strain NK-1 grown on a medium containing starch, Tween 80, and yeast extract. The purified enzyme was monomeric with an Mr 98,000, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and native gel filtration using HPLC. The enzyme had optimal activity with p-nitrophenyl-...

Beet vinasse (BV), a green manure constituted by Trifolium pratense L. uncomposted (TP) and composted with beet vinasse (at 1:1 rate, (TP+BV)1, and 2:1 rate, (TP+BV)2) at 10t organic matter ha(-1) rate were applied during a period of four years for purpose of restoration of a Xelloric Calciorthid located near Seville (Guadalquivir Valley, Andalusia, Spain). The effect on the plant cover, soil p...

The aim of this study was to assess the efficacy of a combination therapy of vildagliptin plus an α-glucosidase inhibitor for patients with type II diabetes mellitus. Type II diabetic patients exhibiting poor glycemic control following α-glucosidase inhibitor treatment for at least two months were selected and randomly distributed into vildagliptin and placebo groups. The body weight, fasting b...

BACKGROUND The filamentous fungus Aspergillus niger is widely exploited as an important expression host for industrial production. The glucoamylase high-producing strain A. niger CICC2462 has been used as a host strain for the establishment of a secretion expression system. It expresses recombinant xylanase, mannase and asparaginase at a high level, but some high secretory background proteins i...

Endoplasmic reticulum (ER) α-glucosidase I is an enzyme that trims the distal α1,2-linked glucose (Glc) residue from the Glc3Man9GlcNAc2 oligosaccharide following its addition to nascent glycoproteins in the initial step of processing. This reaction is critical to the subsequent processing of N-glycans and thus defects in α-glucosidase I gene in human cause congenital disorder of glycosylation ...

BACKGROUND The diagnosis of growth hormone (GH) deficiency (GHD) in short children seems unquestionable when both GH peak in stimulating tests (GHST) and IGF-I concentration are decreased. However, the discrepancies between the results of GHST and IGF-I secretion are observed. It seems purposeful to determine the significance of GHST and IGF-I assessment in diagnosing GHD. The relationship betw...