1. The activities of lysosomal enzymes in the cortexes and medullas and the principal subcellular fractions of rat kidney were measured. 2. A method is described for the isolation of rat-kidney lysosomes and a detailed analysis of the enzymic composition of the lysosomes is reported. Enzyme analysis of the other principal subcellular fractions is included for comparison. 3. Studies of the distr...

Usingdifferent conditions for incubation and fluorometry with 4-methylumbelliferylglycosides as substrates, we demonstratedthe presence ofacida-glucosidase, “renal” a-glucosidase, N-acetyl--D-glucosaminidase A, and N-acetyl-f3-o-glucosaminidase B in freshly drawn normal human serum. The acid a-glucosidase enzymatic activity was determined at pH 4.0 in 0.1 mol/L Tris reagent, whereasthe renal is...

Objective: Alpha-glucosidase, as a carbohydratase catalyses the liberation of alpha-glucose from the nonreducing end of some carbohydrates from foods in digestive tract.  Consequently, retard absorption of glucose by the inhibition of carbohydrate hydrolyzing enzymes is one of the therapeutic approaches to decrease the postprandial hyperglycemia. Recently, a great deal of attention has been pai...

Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the structural gene (malA) encoding the major soluble alpha-glucosidase (maltase) and flanking sequences from Sulfolobus solfataricus were cloned and characterized. This is the first report of an alpha-gl...

Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the structural gene (malA) encoding the major soluble a-glucosidase (maltase) and flanking sequences from Sulfolobus solfataricus were cloned and characterized. This is the first report of an a-glucosidas...

A nitrogen analogue 4 of the naturally occurring sulfonium ion salacinol (1), a potent alpha-glucosidase inhibitor isolated from the Ayruvedic medicine Salacia reticulata, was synthesized and its inhibitory activity against alpha-glucosidase tested. Substitution of the sulfur atom in 1 with a nitrogen reduced the activity considerably. The solid-state stereostructure of the related compound (5)...

application of chemical pesticides has increased significantly worldwide and has raised serious concerns about environmental pollutions. one of the encouraging trends to minimize pesticide risk is production of resistant plants containing toxic proteins against insect pests. considering the importance of purification and characterization of digestive enzymes in the production of resistant plant...

Diabetes is a chronic disease that occurs either when the pancreas does not produce enough insulin or when the body cannot effectively use the insulin. Natural alpha amylase and alpha glucosidase inhibitors from food-grade plants offer an attractive strategy to eather manage or prevent type 2 diabetes by controlling of starch breakdown and intestinal glucose absorption. Black tea is the second ...

Four new pregnane glycosides 1-4 were isolated from the ethanol extract of the stem of Gymnema sylvestre and named gymsylvestrosides A-D. Hydrolysis of compound 1 under the catalysis of Aspergilus niger β-glucosidase afforded compound 5 (gymsylvestroside E). Their structures were determined by spectroscopic methods such as HRESIMS, 1D and 2D NMR, as well as HMQC-TOCSY experiment. Compounds 1-4 ...

a study of insect digestive enzymes makes sense given that the gut is the major interface between the insect and its environment. an understanding of gut and digestive enzyme function is essential when advanced methods of insect management such as application of enzyme inhibitors and transgenic plants are developed to control insect pests. the aim of the current research project was to study mi...