Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis cariniipneumonia, it does not normally stain the cyst wall. For detection ofthe cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining wit...

Aim: Helicobacter pylori infection has been ascertained to play pivotal role in the pathogenesis of chronic gastritis and gastric neoplasia.1The present study was performed evaluate diagnostic accuracy H&E stain Giemsa for histological diagnosis helicobacter by taking immunohistochemical staining as a gold standard. Methods: A total 155 cases were included our study. The received biopsies f...

The karyological features of Akis subtricostata was determined for the first time with conventional and silver nitrate staining. diploid number 2n=16 meioformula 7+neoXY represents a deviation from modal karyotype Coleoptera. pericentromeric heterochromatin detected both Giemsa In addition to determining single possible NOR on prophase I nuclei, AgNO3 revealed that several telomeric regions mit...

Helicobacter pylori is thought to represent a significant etiopathogenic factor in diseases of the upper gastrointestinal tract. It seems, therefore, important to elaborate effective techniques for its detection. The aim of the present study was to evaluate the effectiveness of Helicobacter pylori detection using the PCR technique on paraffin sections with various pairs of primers and to compar...

Background: There are some methods for morphology staining of sperm. Although many different and also new methods are used in semen analysis, light microscopy is still used for routine morphological evaluation. The most used of the methods is Papanicolaou staining. This method is routinely used in Andrology laboratory for Human sperm morphology staining. In some domestic animals such as bull, R...

HeLa 229 cells were infected with genital tract strains of Chlamydia trachomatis. After incubation for varying times the infected cells were fixed and stained with the fluorescent DNA binding dyes Hoechst 33258 or DAPI for comparison with conventional Giemsa stain. Fluorochrome-treated preparations were examined by incident ultraviolet fluorescence microscopy and the Giemsa-stained preparations...

Figure 1. A) Bone marrow aspirate showing numerous Histoplasma capsulatum inside the osteoclastic giant cell and macrophage (inset) (Giemsa, 100 x), B) bone marrow biopsy showing Histoplasma (H&E, 100 x), C) PAS staining showed yeast-like cells with bright eosinophilic structures, D) GMS staining showed clusters of fungal yeasts.

HeLa 229 cells were infected with genital tract strains of Chlamydia trachomatis. After incubation for varying times the infected cells were fixed and stained with the fluorescent DNA binding dyes Hoechst 33258 or DAPI for comparison with conventional Giemsa stain. Fluorochrome-treated preparations were examined by incident ultraviolet fluorescence microscopy and the Giemsa-stained preparations...