The essential oil of Prangos ferulacea (Apiaceae) fruits was obtained by hydrodistillation and analyzed by gas chromatography (GC) and GC-mass spectrometery (MS). Among the 39 identified constituents accounting for 99.99% of the total oil, the major components were chrysanthenyl acetate (26.53%), limonene (19.59%), alpha pinene (19.50%), delta-3-carene (6.56%), mesitaldehyde (6.0...

Mutation of the sesquiterpene synthase Cop2 was conducted with a high-throughput screen for the cyclization activity using a non-natural substrate. A mutant of Cop2 was identified that contained three amino acid substitutions. This mutant, 17H2, converted the natural substrate FPP into germacrene D-4-ol with 77% selectivity. This selectivity is in contrast to that of the parent enzyme in which ...

Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities, was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel enz...

The essential oils of the leaves and fine stems of Pilocarpus microphyllus, collected on iron mineralized soil of the Serra de Caraj s, Southeast of Par State, Brazil, during the rainy and dry seasons, were obtained by hydrodistillation and analyzed by GC-MS. The main identified compounds were 2-tridecanone, beta-caryophyllene, 2-pentadecanone, caryophyllene oxide and germacrene D. Their perce...

the essential oils of peucedanum ruthenicum m. bieb. (umbelliferae) leaves, flowers and fruits were prepared by hydrodistillation and analyzed by gc and gc-ms and the composition of the three essential oils were compared. thirty-three, thirty-seven and thirty-one compounds were identified in leaves, flowers and fruits essential oils representing 89.13%, 88.66% and 76.45% of total oils respectiv...

the essential oil of prangos ferulacea (apiaceae) fruits was obtained by hydrodistillation and analyzed by gas chromatography (gc) and gc-mass spectrometery (ms). among the 39 identified constituents accounting for 99.99% of the total oil, the major components were chrysanthenyl acetate (26.53%), limonene (19.59%), alpha pinene (19.50%), delta-3-carene (6.56%), mesitaldehyde (6.09), and germacr...

The essential oil obtained from the aerial parts of the Turkish endemism Stachys aleurites (Lamiaceae) has been studied. The main constituents were sesquiterpene hydrocarbons: bcaryophyllene (33.7%), bicyclogermacrene (14.5%) and germacrene D (9.6%). The main monoterpene was a-pinene (8.4%). Some chemotaxonomical considerations have been provided. 2004 Elsevier Ltd. All rights reserved.