background: failure in the treatment of burn patients infected with pseudomonas aeruginosa could happen as a result of the acquisition of antibiotic resistance, including carbapenems. objectives: the aim of the present study was to investigate the phenotypic and genotypic characteristics of the pseudomonas aeruginosa strains, isolated from burn patients. patients and methods: during a 12 month ...

morphological and electrophoretic analysis of the crystalline inclusions (parasporal crystals) of sporulated cultures of b. thuringiensis ssp. kumamotoensis dsm 6070 (bt 6070) was conducted via phase contrast and scanning electron microscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. the activity of the spore-crystal suspensions against house fly (musca domestica) larvae wa...

stress is a threatening factor that all living organisms encounter throughout life. depending on the type of stress, there are several mechanisms for keeping body homeostasis to minimize stress effects. brain is an organ which shows high sensitivity to stress conditions. although many studies have shown induced-stress effects on rat embryos, little is known about the mechanisms involved in copi...

background and objective: acinetobacter baumannii is an aerobic non-motile gram-negative bacterial pathogen that is resistant to most antibiotics. carbapenems are the most common antibiotics for the treatment of infections caused by this pathogen. mechanisms of antibiotic-resistance in a. baumannii are mainly mediated by efflux pumps-lactamases. the aim of this study was to determine antibiotic...

a modified pulsed-field gel electrophoresis (pfge) protocol was developed and applied to clinical isolates of mycobacterium tuberculosis complex to reduce the cost of using lyticase. this protocol reduces the expense of pfge typing of mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

conclusions molecular epidemiology studies of infectious diseases have been useful when bacterial isolates have been clustered in a period of time and in different geographical regions with variable antibiotic resistance patterns. in spite of high geographical differences and different antibiotic resistant patterns, low genetic diversity among the studied tb isolates may refer to the low rate o...

4) Briefly describe any new skills you acquired during your summer research: Over the course of the summer, I have learned to optimize many facets of the gel electrophoresis method. I have optimized the construction of a low cost electrophoresis chamber, various tendon cutting techniques, run times, voltage levels, electrode material, and gel analysis techniques. I have also been taught to opti...