The muscarinic acetylcholine receptor of porcine cerebrum has been purified to apparent homogeneity by affinity chromatography, with conjugated 3-(2'-aminobenzhydryloxy)tropane (ABT) as described previously (Haga, K., and Haga, T. (1983) J. Biol. Chem. 258, 13575-13579). In a single step purification using 900 ml of digitonin/cholate-solubilized preparations and 300 ml of the ABT-agarose gel, w...

Ion-pairing high performance liquid chromatography (IP-HPLC) was utilized to monitor the composition changes of blue gel pen ink entries on paper stored in different light conditions and natural environment. The chromatographic conditions were optimized by comparing the separation efficiencies of the blue gel pen inks using a series of ion-pairing reagents, including ammonium carbonate, ammoniu...

Frontispiece: In article number 2000776, Andriy Horechyy, Iryna Yevchuk, Andreas Fery, and co-workers present a series of hybrid proton conducting membranes composed covalently cross-linked interpenetrating polymeric silica networks. The effects monomer composition, sol–gel composition fraction on performance are investigated discussed. These novel hold promise for the application in direct met...

The research is devoted to the development of composition gel-forming mixture and selection percentage components for obtaining intestinal-soluble seamless capsules with probiotics. purpose develop equipment manufacture obtained by drip method. Capsules must have good structural mechanical characteristics, correct shape resistance physical influences, in this regard, it necessary select require...

Spectrophotometric measurements of the Fricke–gelatin–xylenol orange (FGX) gel dosimeter demonstrated reproducible linear dose response up to 25Gy. However, oxidation processes continue post-irradiation, affecting the response of this dosimetry system. Additional oxygenation during preparation increases the sensitivity of the gel but does not improve the auto-oxidation stability of the dosimete...

A double antibody radioimmunoassay (RIA) for rat apolipoprotein A-I is reported. The ApoA-I isolated from delipidated HDL by gel filtration yielded a single band on polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS), and its amino acid composition resembled that reported by others. ApoA-I was iodinated by lactoperoxidase and the resulting 125I-apoA-I was purified by gel filtrati...

We describe a polyacrylamide gel casting cassette that overcomes limitations of commercially available gel electrophoresis equipment. This apparatus molds a single polyacrylamide gel that can evaluate more than 200 samples in parallel, is loaded with a multichannel pipettor, and is flexible with respect to composition of the separating matrix. We demonstrate its use to characterize inhibitors o...

Smooth microsomal cell membranes were isolated by density gradient centrifugation from normal rat liver and from the well-differentiated Morris hepatoma 5123C. The proteins were then extracted from the smooth membrane fraction and compared on acrylamide gel by the split-gel technique and by coelectrophoresis of dually labeled proteins. This analysis revealed the lack of two liver membrane compo...