introduction: g-proteins have an important role in the cell signaling of numerous receptors. the situation of g-proteins in health and disease and their critical role in the development of diabetic side effects is an interested scientific field. here, the changes in the expression of g-protein subunits (gαi, gαs and gβ) were evaluated in hyperglycemic situation of pc12 cells as a cellular model...

mutations in the gjb2 gene are the most common known cause of hereditary congenital hearing loss. rapid genomic dna extraction (rgde) method was used for genomic dna extraction. after amplification of coding region of cx26 gene with specific primers, expected pcr products with 724bp length were subjected to direct sequencing in both directions. we describe here a novel heterozygous -t to -c tra...

Background: DNA amplification method has been developed for identifying and discriminating Salmonella serovars, using specific primers at the genus and serovar levels and to identify the S. Enteritidis, S. Dublin, S. Gallinarum and S. Pullorum. Objectives: This study was conducted for molecular identification and discrimination among some important Salmonella serovars. Methods: Fifty isolates o...

background: giardia duodenalis is one of the most common human intestinal pro-tozoan parasites worldwide and is endemic throughout the world with a vast range of mammalian hosts. the present study aimed to identify the prevalence of g. duo-denalis isolates and determine the most common of its assemblages in the patients referring to health centers and hospitals in fars province, iran that will ...

Lactoferrin is a glycoprotein with molecular weight 80 kDa iron-binding bond, which is composed of 690 amino acids. In most mammalian body fluids such as sweat, semen, tears, and saliva and milk neutrophil granules there. bovine lactoferrin gene be associated with susceptibility/resistance to mastitis and even with some economically important production traits. This study was carried out to det...

an experiment was conducted, using two year old kinnow plants, budded on rough lemon rootstock, inoculated with different isolates of amf fungi viz., glomus manihotis, gigaspora gigantean and glomus mosseae singly as well as in mixed culture. five primers vans1, ns61,ns21,vaglo,vagiga specific to glomus and gigaspora species were designed and used in a polymerase chain reaction with dna extract...

Lactoferrin is a glycoprotein with molecular weight 80 kDa iron-binding bond, which is composed of 690 amino acids. In most mammalian body fluids such as sweat, semen, tears, and saliva and milk neutrophil granules there. bovine lactoferrin gene be associated with susceptibility/resistance to mastitis and even with some economically important production traits. This study was carried out to det...

background the association between tumor necrosis factor-alpha (tnf-α) (-308 g/a) gene polymorphisms and level of tissue destruction and periodontal disease progression is not proved. objectives the present study investigated the stereological parameters of interdental papilla in chronic periodontitis (cp) patients with tnf-α (-308 g/a) gene polymorphisms. materials and methods sixty gingiva sa...

background: campylobacter jejuni and campylobacter coli are zoonotic bacteria which are frequently associated with human diarrhea. sharing of the cytolethal distending toxin (cdt) genes in campylobacter is common and is considered species specific. objectives: in this study we focused on detecting the presence of cdt gene in c. jejuni and c. coli isolated from broilers, turkeys and quails of ir...