We describe an electrofusion-based technique for combinatorial synthesis of individual liposomes. A prototype device with containers for liposomes of different compositions and a fusion container was constructed. The sample containers had fluid contact with the fusion container through microchannels. Optical trapping was used to transport individual liposomes and cells through the microchannels...

Studies using the alphavirus Semliki Forest virus have indicated that the viral E1 fusion protein forms two types of pore: fusion pores and ion-permeable pores. The formation of ion-permeable pores has not been generally accepted, partly because it was not evident how the protein might form these different pores. Here it is proposed that the choice of the target membrane determines whether a fu...

Vacuole fusion requires a coordinated cascade of priming, docking, and fusion. SNARE proteins have been implicated in the fusion itself, although their precise role in the cascade remains unclear. We now report that the vacuolar SNAP-23 homologue Vam7p is a mobile element of the SNARE complex, which moves from an initial association with the cis-SNARE complex via a soluble intermediate to the d...

One of the interesting state-of-the-art approaches to welding is the process of friction stir welding (FSW). In comparison with the fusion processes, FSW is an advantageous method as it is suitable for the non-fusion weldable alloys and polymeric materials joining. Regarding the materials pure solid state joining, it also provides joints with less distortion and enhanced mechanical properties. ...

In the myxomycete Physarum polycephalum the somatic incompatibility between plasmodia of a killer strain and a sensitive strain is expressed as a lethal reaction (LR) in the sensitive strain within 6 h after fusion of both plasmodia. The lethal reaction was not caused by any of the extra-cellular compounds produced by the myxomycete nor was the result of intra-cellular compounds of the killer s...

The fusion reaction of a halo nucleus 11 Be on 208 Pb is described by a three-body direct reaction model. A time-dependent wave packet approach is applied to a three-body reaction problem. The wave packet approach enables us to obtain scattering solutions without considering the three-body scattering boundary conditions. The time evolution of the wave packet also helps us to obtain intuitive un...

The reovirus fusion-associated small transmembrane (FAST) proteins function as virus-encoded cellular fusogens, mediating efficient cell-cell rather than virus-cell membrane fusion. With ectodomains of only approximately 20-40 residues, it is unclear how such diminutive viral fusion proteins mediate the initial stages (i.e. membrane contact and close membrane apposition) of the fusion reaction ...

We investigated the fusion of high-speed liquid droplets as a way to record the kinetics of liquid-phase chemical reactions on the order of microseconds. Two streams of micrometer-size droplets collide with one another. The droplets that fused (13 μm in diameter) at the intersection of the two streams entered the heated capillary inlet of a mass spectrometer. The mass spectrum was recorded as a...

the purpose of this study is the fusion of polarimetric sar and hyperspectral images for concealed target detection in forest areas. the proposed method is using the polarimetric and the spectral signatures. a radarsat-2 image and a hyperion image of the city san francisco were chosen for evaluation of the proposed method. the four targets were selected in the forest and the outer areas. three ...