The application of high-throughput, next-generation sequencing technologies has greatly improved our understanding of the human oral microbiome. While deciphering this diverse microbial community using such approaches is more accurate than traditional culture-based methods, experimental bias introduced during critical steps such as DNA extraction may compromise the results obtained. Here, we sy...

Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve experimental results. In order to optimize a protocol for obtaining high purity DNA from soil microbio...

Background: Dermatophytes are the most common fungal agents causing superficial skin infections in worldwide. Species identification of these fungi is important for therapeutic and epidemiological apects. The purpose of this study was to determine the epidemiology of dermatophytosis in patients referring to medical mycology laboratory of Razi hospital in Tehran, during 2014. Materials and Meth...

Molecular identification techniques based on total DNA extraction provide a unique tool for identification of mycelium in soil. Using molecular identification techniques, the ectomycorrhizal (EM) fungal community under coniferous vegetation was analyzed. Soil samples were taken at different depths from four horizons of a podzol profile. A basidiomycete-specific primer pair (ITS1F-ITS4B) was use...

Five commercially available extraction kits and an in-house DNA extraction method for the release of DNA from Candida albicans and Aspergillus niger cells were assessed for sensitivity, purity, duration, and cost. All commercially available kits helped to shorten the duration of DNA extraction. However, the sensitivity varied from 1 to 1,000 fungal cells/ml and costs varied from $0.10 to 2.30. ...

Background & Objectives: Fungal infections of the eye are rampant in tropical regions and are responsible for significant ocular morbidity and blindness. Prompt and specific identification of fungi is important in order to define clinical treatment. However, in most cases conventional culture identification can be considered to be time consuming, of low sensitivity and not without errors. The a...

The expediency of using unique SSR DNA-markers Schizophyllum commune for population genetic assays in various fungal species has been demonstrated. In Auricularia auricula-judae and Irpex lacteus, we observed formation heterogeneous amplicons mostly up to 500 bp length that ensured their high resolution facilitated data analysis. established sets molecular markers are efficient DNA-fingerprinti...

Fungal pure cultures identified with both classical morphological methods and through barcoding sequences are a basic requirement for reliable reference sequences in public databases. Improved techniques for an accelerated DNA barcode reference library construction will result in considerably improved sequence databases covering a wider taxonomic range. Fast, cheap, and reliable methods for obt...

Aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecologicalniches and the most prevalent aspergilli responsible for human and animal invasiveaspergillosis. The first step to develop novel and efficient therapies is the identificationand understanding of the key tolerance and virulence factors of pathogens. The mainfocus of the present study is to perform the similarit...

Agrobacterium tumefaciens-mediated transformation (ATMT) has become a prevalent tool for functional genomics of fungi, but our understanding of T-DNA integration into the fungal genome remains limited relative to that in plants. Using a model plant-pathogenic fungus, Magnaporthe oryzae, here we report the most comprehensive analysis of T-DNA integration events in fungi and the development of an...