The eukaryotic RNA exosome is a highly conserved multi-subunit complex that catalyzes degradation and processing of coding and noncoding RNA. A noncatalytic nine-subunit exosome core interacts with Rrp44 and Rrp6, two subunits that possess processive and distributive 3'-to-5' exoribonuclease activity, respectively. While both Rrp6 and Rrp44 are responsible for RNA processing in budding yeast, R...

Harvest time and storage had significant effects on nutrient quality Volatile Organic Compounds (VOCs) of alfalfa. The objective this study was to use headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) analyze alfalfa at different harvest periods (budding stage, early blooming, full blooming), for 0 d, 90 180 270 360 the dynamic changes in VOCs nutritiona...

The human protein kinase ULK3 regulates the timing of membrane abscission, thus being involved in exosome budding and cytokinesis. Herein, we present first high-resolution structures domain. Its unique features are explored against background other ULK kinases. An inhibitor fingerprint indicates that is highly druggable capable adopting a wide range conformations. In accordance with this, descr...

About 500 species of wormwood are known in the world. In flora Azerbaijan, this genus is represented by 42 taxa. The main chemicals shown to be sesquiterpene lactones, coumarins, steroids, and essential oils. From aerial parts Artemisia campestris (var. Marschalliana) Spreng., collected during phenological phase budding, an acetone extract was obtained, which, after removal solvent, subjected c...

Herpesvirus capsids originating in the nucleus overcome the nucleocytoplasmic barrier by budding at the inner nuclear membrane. The fate of the resulting virions is still under debate. The fact that capsids approach Golgi membranes from the cytoplasmic side led to the theory of fusion between the viral envelope and the outer nuclear membrane, resulting in the release of capsids into the cytopla...

Nitrogen (N) fertilizers such as urea are applied to peach orchards worldwide whenever the soil cannot meet plants’ N demand. However, actual nutritional contribution of different supply modes crops, or residual in subsequent cycle, is yet be fully known. The current study aims assess annual and nutrition trees grown under subtropical climate. Forty kilograms per hectare supplied enriched (3.0 ...

The hepatitis B virus (HBV) is an enveloped DNA virus that replicates via reverse transcription of its pregenomic RNA (pgRNA). Budding of HBV is supposed to occur at intracellular membranes and requires scission functions of the endosomal sorting complex required for transport (ESCRT) provided by ESCRT-III and VPS4. Here, we have investigated the impact of the upstream-acting ESCRT-I and ESCRT-...

Retroviruses acquire a lipid envelope during budding from the membrane of their hosts. Therefore, the composition of this envelope can provide important information about the budding process and its location. Here, we present mass spectrometry analysis of the lipid content of human immunodeficiency virus type 1 (HIV-1) and murine leukemia virus (MLV). The results of this comprehensive survey fo...