semen quality and its relationship to fertility are major concern in animal production. the aim of this study was to assess the relationship between frozen thawed sperm characteristics and fertility following in vivo fertility through artificial insemination(ai). semen samples were collected from four buffalo bulls. semen volume and sperm concentration appeared to be significantly different (p0...

Introduction The procedure for artificial insemination (AI) in pigs using cryopreserved semen involves the deposition of a high number of cryopreserved spermatozoa (5-6 x10 cells) in a large volume of diluent (80-100 ml) into the cervix. Despite inseminating with this high sperm number, low fertility levels are usually obtained (about 20-30% conception and farrowing rates and 2-3 total piglets ...

Bovine herpesvirus 1 (BoHV-1) and 5 (BoHV-5) are important pathogens of the respiratory and genital tract of cattle and may also affect the central nervous system and cause meningoencephalitis. Both virus types are estimated to be widely distributed in Southern Brazil. In the present study, BoHV-1 and/or BoHV-5 DNA were detected in bovine semen samples from two states of Brazil by two species-s...

This study was conducted to assess the post thaw quality of Kundhi buffalo semen diluted in tris based diluents. After collection the ejaculates were pooled and checked for color, volume, pH, mass activity, progressive linear motility% (PLM), and intactness of cell membrane. The semen qualifying these tests was divided into four aliquots A, B, C and D, diluted with tris based diluents; the last...

OBJECTIVE To evaluate the spermatotoxicity of gold nanoparticles. DESIGN Experimental study. SETTING A university in Thailand. PATIENT(S) Single-donor fresh semen sample. INTERVENTION(S) A mixture of gold nanoparticle solution and semen was prepared and further analyzed. MAIN OUTCOME MEASURE(S) Spermatozoa appearance. RESULT(S) After mixing the semen with a gold nanoparticle solutio...

The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg mL-1 sodium selenite and the sperm motility and...

Flow cytometry is considered the only reliable method for the separation of X and Y chromosome bearing spermatozoa in equines. The MoFlo SX DP sorter is highly efficient, allowing the production of foals of the desired sex. However, to achieve acceptable pregnancy rates the currently used protocol requires working with fresh semen obtained close to, or at, the sorting facility. An alternative p...

BACKGROUND Standard semen parameters have proven poor at predicting the outcomes of IVF treatment cycles. As recent studies suggest that the male genome may play an important role in early embryogenesis, this study attempts to correlate the level of sperm DNA damage in fresh semen and prepared sperm with the outcomes of conventional IVF treatment cycles. METHODS Forty patients embarking on IV...

Introduction The interest for canine semen preservation and artificial insemination (AI) is steadily increasing worldwide, yet comparatively few studies are published each year within this field, and it is particularly difficult to find data from artificial inseminations using fresh semen, or semen preserved according to various methods for chilling, or freezing and thawing, and also comparing ...