The freezing/thawing stratification effect of seasonal factors or artificial disturbances in frozen soil regions has an important influence on the vertical vibration pile–soil–cap system. Taking into account slippage between pile and soil, a simplified layered analytical model system double-layered stratum under freezing thawing processes saturated porous medium was established, solution dynami...

Using a freezing-thawing method for extracting colicine from solid media it has been shown that the choice of media for production and diffusion is important. Digest nutrient agar yielded the most colicine and peptone water agar the least. A factor in bacteriological peptone, but absent in a proteose peptone (Difco) and Neopeptone, was responsible for inhibiting production on peptone water agar...

The objective of this study was to test the hypothesis that human adipose tissue-derived adult stem cells (ASCs) can be effectively cryopreserved and stored in liquid nitrogen using a freezing medium containing a high-molecular-weight polymer, polyvinylpyrrolidone (PVP), as the cryoprotective agent (CPA) instead of dimethylsulfoxide (DMSO). To this end we investigated the postfreeze/thaw viabil...

Objective: Mammalian spermatozoa swell under hypotonic conditions. The exposure of spermatozoa to isotonic conditions after exposure to hypertonic conditions during cryopreservation results in the occurrence of osmotic shock, which is characterized by coiling of the distal end of the sperm tail. The objective of this study was to assess the effects of osmotic shock (% sperm coiling) on human cr...

The wood frog ( Rana sylvatica) is a freeze-tolerant species that encounters subzero temperatures during its winter breeding season, whereas the leopard frog ( R. pipiens) is freeze intolerant and breeds in spring. Osmotic and freezing tolerances of spermatozoa from these species were inferred from spermolysis rate, integrity of the plasma membrane as judged using vital dye assay, and motility ...

[1] I examine the morphology of ice growth in porous media. Intermolecular forces cause premelted fluid to migrate and supply segregated ice growth (e.g., lenses) and frost heave. I account for the net effect of these microscopic interactions in a homogenized model formulated in terms of fundamental physical properties and characteristics of the porous medium that can be measured; no ad hoc par...