5-Hydroxymethylcytosine (5hmC) is a modified base present at low levels in various mammalian cells, and it plays essential roles gene expression, DNA demethylation, genomic reprogramming. Herein, we develop label-free template-free chemiluminescent biosensor for sensitive detection of 5hmC DNAs based on 5hmC-specific glucosylation, periodate (IO4+) oxidation, biotinylation, terminal deoxynucleo...

In Escherichia coli, the gyrase enzyme is composed of two subunits: A and B [1]. The A subunit (97 k Da) interacts and relieves DNA super coils through its active site (tyrosine) during DNA replication. The B subunit contains the ATP as active site required to release the energy in the attached ATP molecule and provides the free energy to the reaction (DNA super coiling) accomplished by the gyr...

BACKGROUND Cell-free fetal DNA and cell-free total DNA in maternal circulation have been proposed as potential markers for noninvasive monitoring of the placental condition during the pregnancy. However, the correlation of and change in cell-free fetal DNA and cell-free total DNA in spontaneous abortion (SA) with fetal chromosomal aneuploidy have not yet been reported. Therefore, we investigate...

recently, several studies have shown that the metal-fluoroquinolone complexes have more antibacterial and cytotoxic effects in comparison with free fluoroquinolones. these results may introduce new drugs for chemotherapy with fewer side effects. in this work a bidentated zinc (ii) complex with ofloxacin (ozc) was synthesized and cytotoxicity activities and dna binding of the resulted complex wa...

Free-radical metabolites can be generated metabolically by a one-electron reductase-catalyzed reaction or a "peroxidase" catalyzed oxidation or by photoactivation of a wide variety of aromatic xenobiotics. Radicals may also be generated during lipid peroxidation. Some radicals can react with DNA or bind covalently or noncovalently as a dismutation product or as a dimer, trimer or polymeric prod...

In 1947, Mandel and Métais discovered the unusual phenomenon of circulating cell-free DNA (1 ). Although one might naively think that DNA is found only in the nuclei of cells, it turns out that a substantial amount of small DNA fragments circulates within the blood. These molecules are not part of intact cells but are the detritus of dead and apoptosed cells that have spilled their guts into th...

heterogeneous and dynamic problems of engineering technology and systems for industry and government. ISR is a permanent institute of the University of Maryland, within the Glenn L. Martin Institute of Technology/A. James Clark School of Engineering. It is a National Science Foundation Engineering Research Center. Web site http://www.isr.umd.edu I R INSTITUTE FOR SYSTEMS RESEARCH UNDERGRADUATE ...

Objective: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. Materials and Methods: HepG2 were incubated with irinotecan (100 µM), heat-killed cells (0.025 µg/ml) + irinotecan...