Since Ledbetter and Porter (1964) described the 13 subunits which are visible in cross sections of negatively stained plant microtubules, subsequent observations have generally confirmed this number. By using Mizuhira's fixative composed of tannic acid and glutaraldehyde, it is easyto demonstrate the subunits of microtublules without optical reinforcement Cytoplasmic microtubules and sperm axon...

Chromosomes in the nuclei of living salivary glands of Simulium have been observed with Nomarski optics and polarized light before and during irrigation with various fixatives. All cause loss of chromosomal organization to some degree. The best fixative is redistilled glutaraldehyde; even so, although it leaves the nucleus visually unaltered, a network forms in the nuclear sap and birefringence...

The synthesis and characterization of a novel fixative, guar gum-graftpoly(acrylamide-co-diallyldimethylammonium chloride) (GG-g-p(AM-coDADMAC)) polymer is described. The grafted polymer proved to be effective at adsorbing hydrophobic wood resin particles onto papermaking fibre surfaces, thus removing wood resins from the water phase where they have a tendency to aggregate and form troublesome ...

An essential to the study of the histiogenesis of leprosy consists of reliable demonstrations of the bacilli in tissues. In the older days, when celloidin embedding was more widely used, fair results were often seen. Paraffin embedding, however, has almost totally replaced celloidin in common practice, and the problem has become that of determining the conditions of fixation, dehydrating, and s...

TESTING OF SACCHAROMYCES CEREVISIAE MORPHOLOGICAL FIXATIVES AND FIXED SAMPLES STORED AT AMBIENT TEMPERATURE Diana Ly, Diane Yu, Beverly Girten and Jacob Cohen Education Associates Program (University of San Francisco), Lockheed Martin Space Operations, NASA Ames Research Center, Science and Technology Corporation, Moffett Field, CA EMMYS-1, Effects of Microgravity on Model Yeast Specimen, is a ...