Introduction: Isolation of fungi from tissue specimens using conventional methods is time consuming. However, in some cases, the histopathological examination (HPE) alone unable to provide a definite identity fungus. Alternatively, non-culture method, such as polymerase chain reaction (PCR) detecting internal transcribed spacer (ITS) rRNA genes fungi, promising diagnostic tool for rapid and acc...

background and objective: formalin-fixed paraffin-embedded tissues are a valuable source of dna for molecular studies. we designed and optimized an efficient procedure for dna extraction from formalin-fixed paraffin embedded tissues. materials and methods: seventy three blocks of cervical paraffin-embedded tissues were investigated. dna was extracted using 45 minutes boiling in alkaline solutio...

Proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue would enable retrospective biomarker investigations of this vast archive of pathologically characterized clinical samples that exist worldwide. These FFPE tissues are, however, refractory to proteomic investigations utilizing many state of the art methodologies largely due to the high level of covalently cross-linked proteins ...

MALDI Imaging is an analytical method for the detection of potential biomarkers directly from tissue sections that has gained popularity over the past decade. The development protocols for spatially resolved digests are of particular interest. Most importantly, on-tissue digestion allows the analysis of peptides from formalin-fixed paraffin-embedded (FFPE) tissue, the most common type of sample...

Formalin-fixed paraffin-embedded (FFPE) breast tumor tissues are readily available and represent a largely untapped, vast resource for molecular profiling of clinical samples with long-term follow-up data. We have optimized the conditions and parameters that result in the preparation of total RNA that is of the necessary quality for use in the DASL (cDNA-mediated annealing, selection, extension...

The large archives of formalin-fixed paraffin-embedded (FFPE) tissue specimens that exist are a highly valuable source of sample material for molecular biological analysis, including gene expression profiling. However, current data on adverse effects of standard pathological practice on the usefulness of biomolecular analytes obtained from such archived specimens is largely anecdotal. Here, we ...

Formalin-fixed paraffin-embedded (FFPE) tissue samples represent a potentially invaluable resource for transcriptomic research. However, use of FFPE samples in genomic studies has been limited by technical challenges resulting from nucleic acid degradation. Here we evaluated gene expression profiles derived from fresh-frozen (FRO) and FFPE mouse liver tissues preserved in formalin for different...

Title: Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded tissue (FFPE) for PCR-based methylation analysis using SMART-MSP and MS-HRM Version: 2 Date: 30 October 2009 Reviewer: Catherine Gratas Reviewer's report: The authors answered to most of my comments and improved their paper. Discretionary revisions: 1The number of unmethylated tumors and negative b...

This study investigated the use of pressure cooking with different retrieval solutions at variable pHs to determine the optimum protocol for the extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissues. Further, the oxidation effect on the archived FFPE tissues in relation to the age of the tissue blocks was also explored. The main aim of the study was to investigate ...