Fixation is a critical step in the preparation of tissues for histopathology. The objective of this study was to investigate the effects of different fixatives vs formalin on proteins and DNA, and to evaluate alternative fixation for morphological diagnosis and nucleic acid preservation for molecular methods. Forty tissues were fixed for 24 h with six different fixatives: the gold standard fixa...

The emission of ultrasonic vocalization (USV) by rats submitted to the formalin test has not yet been demonstrated. We performed two experiments to establish the formalin concentration to induce USV and the relationship of USV emission with motor behaviors and the effects of morphine and naloxone on USV during the formalin test. Male Wistar rats were used. In Experiment 1, 3 different groups of...

BACKGROUND Formalin is widely used to fix histological preparations and as preservatives in embalming solutions and is an age-long practice in medical laboratories. It is generally accepted that the risk of contracting infections is relatively high among medical laboratory workers and pathologists. Recent studies have, however, suggested that formalin does not effectively inactivate all kinds o...

Formalin merupakan bahan kimia yang digunakan sebagai pengawet dalam makanan. Bahan ini bersifat karsinogenik, akumulasi tinggi tubuh akan menyebabkan iritasi lambung, alergi sampai terjadinya kanker saluran cerna. Jenis makanan di pasar diantaranya ayam potong, ikan basah, cumi, kerang, sotong, udang, asin, otak-otak, bakso dan mi basah dengan sengaja menggunakan formalin pengawet. Tujuan peng...

Histopathology is the most useful tool for diagnosis of a number of diseases, especially cancer. To be effective, histopathology requires that tissues be fixed prior to processing. Formalin is currently the most common histologic fixative, offering many advantages: it is cheap, readily available, and pathologists are routinely trained to examine tissues fixed in formalin. However, formalin fixa...

OBJECTIVE(S) There are many reports about the role of rostral ventromedial medulla (RVM) in modulating stress-induced analgesia (SIA). In the previous study we demonstrated that temporal inactivation of RVM by lidocaine potentiated stress-induced analgesia. In this study, we investigated the effect of permanent lesion of the RVM on SIA by using formalin test as a model of acute inflammatory pai...

abstract background: occupational exposure studies indicate that formaldehyde exposure causes temporary and consistent effects on industrial workers exposed to formalin. case: the case was a 36-year-old man who had developed intravascular hemolytic anemia caused by formalin after inhalation exposure. formalin is a clear solution of 37% formaldehyde in water. the primary route of exposure to for...

the leukotrienes and prostaglandins are biologically active metabolites derived from arachidonic acid. the leukotrienes have a role in inflammatory diseases such as allergic rhinitis, inflammatory bowel disease and asthma. montelukast, a cysteinyl leukotriene receptor antagonist, is claimed to be effective in asthma. the present study aimed to assess the role of cysteinyl leukotriene receptor a...

introduction: the management of pain and inflammation related problem is a real challenge that people face daily. although several drugs are available for these conditions, medicinal plants are believed to be an important source of new chemical substances with potential therapeutic effects. the objective of current study was to investigate the anti-nociceptive effect of tanacetum fisherae which...

Gabapentin and baclofen at doses not affecting motor performance, produced dose-dependent inhibition of both phases in the formalin test in mice. Isobolographic analysis revealed an additive interaction between the studied drugs in the second phase of the formalin test. Gabapentin given at doses effective in both phases of the formalin test significantly potentiated baclofen-induced motor impai...