نتایج جستجو برای: fluorometric method

تعداد نتایج: 1631856  

Journal: :Analytical biochemistry 2005
Wei-Ti Chen Ming-Chih Liu Yuh-Shyong Yang

A sensitive fluorometric assay was developed for alcohol sulfotransferase (AST). This was the first continuous fluorometric assay reported for AST. It used 3'-phosphoadenosine 5'-phosphosulfate regenerated from 3-phosphoadenosine 5'-phosphate by a recombinant phenol sulfotransferase (PST) using 4-methylumbelliferyl sulfate as the sulfuryl group donor. The recombinant PST did not use the alcohol...

Journal: :Clinical chemistry 1973
H K Lau G G Guilbault

Measurement of serum creatine kinase (CK; EC 2.7.3.2) activity is of great value in the diagnosis of myocardial and skeletal muscle diseases (1-3). The greater specificity of CK over that of other enzymes, such as lactate dehydrogenase and aspartate aminotransferase, for the detection of muscular dystrophy and myocardial infarction has attracted much interest in CK determinations. In a prelimin...

Journal: :The Journal of biological chemistry 1949
M GORDON H K MITCHELL

The various calorimetric methods now employed for the estimation of tryptophan are not specific for tryptophan but give colored products with many compounds containing the indole nucleus, including indole itself. In order to facilitate studies of the enzymatic synthesis of tryptophan from indole and serine by extracts of Neurospora (l), a search for a rapid, quantitative method for the estimati...

Journal: :Clinical chemistry 1975
J C Valentour J R Monforte B Lorenzo I Sunshine

We report a fluorometric method for detecting diazepam, chlordiazepoxide, oxazepam, chlorazepate, and (or) their major metabolites in blood, urine, or gastric contents at low therapeutic concentrations. The drugs are first hydrolyzed to their respective benzophenones and converted to highly fluorescent 9-acridanones. Total benzodiazepines (parent plus metabolites) in blood and gastric contents ...

Journal: :Clinical chemistry 1967
B Searle M B Mijuskovic D Widelock B Davidow

THE MICROBIOLOGIC PROCEDURE of Guthrie and Susi (1) is probably the most widely used screening technic for detecting phenylketonuria. The method of McCaman and Robins (2) and its modifications by Wong et al. (3) and Hsia et at. (4), as well as that of La Du and Michael (5) are the chemical procedures generally used as confirmatory tests. Hill et at. (6) have adapted the McCaman procedure to the...

Journal: :Clinical chemistry 1969
J A Ambrose

As a result of a study of the classic ninhydrin reaction with amino acids (which results in the productionof Ruhemann’spurple), and the fluorescencereaction occurring between ninhydrin and phenylalanine in the presence of a dipeptide, the initial incubation period in the fluorescence reaction was shortened from 2 hr. to 16 mm. This improvement in the fluorometric method for the determination of...

2003

A number of chemical methods have been described for the determination of riboflavin (1-13). These methods are in general limited in use because, when applied to certain products, either they are not specific for riboflavin or all the riboflavin present is not recovered (2). In most cases the authors of papers presenting riboflavin methods have indicated that the methods are of limited applicat...

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