Much effort has been expended in the measurement of the fluorescence decay kinetics of chlorophyll in vioo (for examples see papers cited in Harris et al., 1976). To the present time most techniques have lacked either the precision or the time resolution required for accurate determinations. Recent work using high-powered lasers has been hampered asa result of anomalousmultiphotoneffects(Campil...

Deazanebularin, a cytotoxic nucleoside, is fluorescent at ambient temperature and at neutral pH. Some of its fluorescence properties and those of its phosphorylated derivatives are reported here. Deazanebularin nucleotides are substrates for Escherichia coli polynucleotide phosphorylase and RNA polymerase. With RNA polymerase deazanebularin triphosphate acts as an ambiguous substrate in that it...

The reactions of 2-chloropyrimidine with methylamine, ethylamine and piperidine gave the corresponding 2-N-methylamino-, 2-N-ethylamino- and 2N- piperidinopyrimidines, respectively. The fluorescence properties of these alkylamino derivatives in chloroform, ethyl acetate, carbon tetrachloride, acetone, ether, ethanol and methanol were studied. All the alkylamino derivatives showed the highest fl...

LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...

The generally used model for rotational diffusion, the Perrin-Stokes-Einstein model [l-3] , is based on hydrodynamics with the “stick” boundary condition. That is, the velocity of the solute molecule surface relative to the nearest solvent molecules is zero. Recently Hu and Zwanzig [4] have derived rotational friction coefficients for spheroids with the “slip” boundary condition. Here, the resi...

objective(s):the objective of this study was to investigate the interaction of daunorubicin (dnr) and cardiac myosin (cm) and the changes in mice hearts to exhibit dnr-induced cardiotoxicity . materials and methods:the interaction between dnr and cm was expressed using fluorescence quenching at ph 4.0-9.0 and 15-37 °c. dnr-induced cardiotoxicity was studied using in vivo experiment.  forty grou...

Background: Chlamydomonas reinhardtii is a novel recombinant eukaryotic expression system with many advantages including fast growth rate, rapid scalability, absence of human pathogens and the ability to fold and assemble complex proteins accurately, however, obstacle relatively low expression level necessitates optimizing foreign gene expression in this system. The Green Fluorescent Protein (G...

Background: Ethnomedicinally, the plant Argyreia pilosa Wight & Arn. (Convolvulaceae) has long been utilized in various disorders in the conventional system; most significantly it is utilized against sexually transmitted diseases, skin troubles, diabetes, rheumatism, cough, and quinsy. The key challenge experienced in the standardization of herbal drugs is the lack of proper identification of p...