The transversal distribution of phosphatidylcholine, phosphatidylethanolamine, and cardiolipin in the inner membrane of beef heart mitochondria was investigated. Highly purified “right-side-out” (mitoplasts) and “inside-out” (submitochondrial particles) membranes were used, permitting the independent study of the phospholipid composition on either half of the membrane. Three independent, and pa...

A new sensitive analytical procedure for the determination of albumin is presented, based on the participation of a fluorescent derivative of the protein in the peroxyoxalate chemiluminescent system using imidazole as a catalyst. The chemiluminescent emission is detected in a flow injection analysis (FIA) system, employing micellar medium as carrier, whereas the derivatisation reaction is carri...

The free primary amines present in the faeces of 44 infants (1-18 months) with gastroenteritis have been examined by field desorption mass spectrometry of the lactone form of their fluorescamine derivatives without their prior separation. p-Tyramine, 2-phenylethylamine, the diamines, putrescine and cadaverine and several of their acyl derivatives were common constituents, but a number of other ...