A stationary bubble-swarm has been used to aerate a mammalian cell culture bioreactor with an extremely low gas flow rate. Prolonging the residence time of the gas bubbles within the medium improved the efficiency of the gas transfer into the liquid phase and suppressed foam formation. An appropriate field of speed gradients prevented the bubbles from rising to the surface. This aeration method...

Solar reactors featuring a circulating cyclone flow pattern provide enhanced heat transfer and longer residence time increasing the conversion efficiency. Cyclone flow also works in reducing particle deposition on solar reactor walls and exit, which is particularly an important issue in solar cracking reactors to avoid clogging. This paper focuses on the physics of cyclone formation inside a so...

The paper is focused on the comparison of the reverse flow reactor (RFR) and of the counter-current reactor (CCR) operation, focusing the attention on the interaction between transport phenomena and chemical reactions, as well as on the analysis of the dynamic behavior. A heterogeneous mathematical model was used in order to investigate the performance of both reactor configurations. The role o...

BACKGROUND Saccharomyces cerevisiae BY4741 is an auxotrophic commonly used strain. In this work it has been used as host for the expression and secretion of human interleukin-1beta (IL1beta), using the cell wall protein Pir4 as fusion partner. To achieve high cell density and, consequently, high product yield, BY4741 [PIR4-IL1beta] was cultured in an aerated fed-batch reactor, using a defined m...

BACKGROUND Corynebacterium glutamicum has large scale industrial applications in the production of amino acids and the potential to serve as a platform organism for new products. This means the demand for industrial process development is likely to increase. However, large scale cultivation conditions differ from laboratory bioreactors, mostly due to the formation of concentration gradients at ...

Following a thorough site investigation, a biological Sequential Reactive Barrier (SEREBAR), designed to remove Polycyclic Aromatic Hydrocarbons (PAHs) and BTEX compounds, was installed at a Former Manufactured Gas Plant (FMGP) site. The novel design of the barrier comprises, in series, an interceptor and six reactive chambers. The first four chambers (2 nonaerated-2 aerated) were filled with s...

A mathematical model of a mixed culture of bacteria in a fully three dimensional flow reactor which accounts for the colonization of the reactor wall surface by the microbes is studied both analytically and by simulation. It can be viewed as a model of the large intestine or of the fouling of a commercial bio-reactor or pipe flow. The primary focus is on coexistence of microbial populations in ...