*Address correspondence to: A.J. Jiménez. Departamento de Biología Celular y Genética, Facultad de Ciencias, Universidad de Málaga, Spain. e-mail: [email protected] ABSTRACT Hyh is a lethal autosomal recessive mouse mutation located in chromosome 7. Newborn homozygous mutants presented moderate hydrocephalus, and then a severe hydrocephalus develops during the first postnatal days (PérezFígares ...

The dorsal column-medial lemniscal system is a significant sensory pathway that mediates touch and limb position sense. In this system, axons from the second-order neurons in the dorsal column nuclei form the internal arcuate fibers, cross the ventral midline (floor plate) within the medulla oblongata, and then project to the thalamus as the medial lemniscus. Here we demonstrate that Netrin-1, ...

During early neurogenesis, the floor plate plays an essential role(s) in the differentiation of the ventral portion of neural tube. In this study, we detected the specific distribution of unique glycoconjugate during the floor plate differentiation. Formalin fixed paraffin sections of BALB/c mice (10-14 embryonic days) were processed for histochemical studies using horseradish peroxidase-labell...

During early neurogenesis, the floor plate plays an essential role(s) in the differentiation of the ventral portion of neural tube. In this study, we detected the specific distribution of unique glycoconjugate during the floor plate differentiation. Formalin fixed paraffin sections of BALB/c mice (10-14 embryonic days) were processed for histochemical studies using horseradish peroxidase-labell...

The thickness of the ventral midline of the spinal cord was determined in 9 human embryos aged five weeks (developmental stages 13-15). This part of the spinal cord consists of floor plate, mantle and marginal layers. The floor plate ependymal cells form pseudostratified columnar epithelium. The thickness of the investigated structure varied from 20 to 50 micrometers at different levels of the ...

Cilia are at the core of planar polarity cellular events in many systems. However, the molecular mechanisms by which they influence the polarization process are unclear. Here, we identify the function of the ciliopathy protein Rpgrip1l in planar polarity. In the mouse cochlea and in the zebrafish floor plate, Rpgrip1l was required for positioning the basal body along the planar polarity axis. R...

Sonic hedgehog (Shh) is a secreted protein that is involved in the organization and patterning of several tissues in vertebrates. We show that the zebrafish sonic-you (syu) gene, a member of a group of five genes required for somite patterning, is encoding Shh. Embryos mutant for a deletion of syu display defects in patterning of the somites, the lateral floor plate cells, the pectoral fins, th...

We analysed the role of Notch signalling during the specification of the dorsal midline in Xenopus embryos. By activating or blocking the pathway we found that Notch expands the floor plate domain of sonic hedgehog and pintallavis and represses the notochordal markers chordin and brachyury, with a concomitant reduction of the notochord size. We propose that within a population of the early orga...

During development, precerebellar neurons migrate dorsoventrally from the rhombic lip to the floor plate. Some of these neurons cross the midline while others stop. We have identified a role for the slit receptor Rig-1/Robo3 in directing this process. During their tangential migration, neurons of all major hindbrain precerebellar nuclei express high levels of Rig-1 mRNA. Rig-1 expression is rap...