The GTPase dynamin assembles at the necks of budded vesicles in vivo and functions in membrane fission. We have developed fluid supported bilayers with excess membrane reservoir, (SUPER) templates, to assay vesicle formation and membrane fission. Consistent with previous studies, in the absence of GTP, dynamin assembles in spirals, forming long membrane tubules. GTP addition triggers disassembl...

The crypts of the intestinal epithelium house the stem cells that ensure the continual renewal of the epithelial cells that line the intestinal tract. Crypt number increases by a process called crypt fission, the division of a single crypt into two daughter crypts. Fission drives normal tissue growth and maintenance. Correspondingly, it becomes less frequent in adulthood. Importantly, fission i...

Many gregarious species display rapid fission-fusion dynamics with individuals frequently leaving their groups to reunite or to form new ones soon after. The adaptive value of such ephemeral associations might reflect a frequent tilt in the balance between the costs and benefits of maintaining group cohesion. The lack of information on the short-term advantages of group fission, however, hamper...

When an agent undergoes fission, how should the beliefs of the fission results relate to the pre-fission beliefs? This question is important for the Everett interpretation of quantum mechanics, but it is of independent philosophical interest. Among other things, fission scenarios demonstrate that “self-locating” information can affect the probability of uncentred propositions even if an agent h...

Mitochondria undergo cycles of fusion and fission crucial for organelle homeostasis. Fission is regulated partially by recruitment of the large GTPase Dnm1p to the outer mitochondrial membrane. Using three-dimensional time-lapse fluorescence imaging of Saccharomyces cerevisiae cells, we found that Dnm1p-EGFP appears and disappears at “hot spots” along mitochondrial tubes. It forms patches that ...

Rationale: Airway epithelial cells (AECs) are critical regulators of inflammatory, immune and injury responses to allergens that contribute to asthma pathogenesis. The response of AECs to allergens requires an integrated-complex, extracellular receptors and intracellular organelle interaction to achieve secretion of pro-inflammatory cytokines and chemokines. Endoplasmic reticulum (ER) and mitoc...

Since its discovery 75 years ago fission has been at the forefront of nuclear science and engineering research. Although the fission process is being used worldwide to generate safe reliable power, there are several aspects of the fission process which are still not well known. One such parameter of the fission process is the prompt fission neutron spectrum (PFNS). Although the average number o...

The current status of the application of covariant density functional theory to microscopic description of nuclear fission with main emphasis on superheavy nuclei (SHN) is reviewed. The softness of SHN in the triaxial plane leads to an emergence of several competing fission pathes in the region of the inner fission barrier in some of these nuclei. The outer fission barriers of SHN are considera...

Mitochondria are dynamic organelles, undergoing both fission and fusion regularly in interphase cells. Mitochondrial fission is thought to be part of a quality-control mechanism whereby damaged mitochondrial components are segregated from healthy components in an individual mitochondrion, followed by mitochondrial fission and degradation of the damaged daughter mitochondrion. Fission also plays...

The yeast protein Fis1p has been shown to participate in mitochondrial fission mediated by the dynamin-related protein Dnm1p. In mammalian cells, the dynamin-like protein DLP1/Drp1 functions as a mitochondrial fission protein, but the mechanisms by which DLP1/Drp1 and the mitochondrial membrane interact during the fission process are undefined. In this study, we have tested the role of a mammal...