This study comprises 12 corneal buttons removed at the time of keratoplasty from patients with bullous keratopathy due to Fuchs' dystrophy. All except one were aphakic. Three had vitreous contact to the endothelium. One patient had several intraocular procedures. Specimens were processed for scanning electron microscopy and dried in a freeze-drying or critical-point machine. Portions of these s...

An on-line and continuous technique based on electric cell-substrate impedance sensing (ECIS) was developed for measuring the concentration and time response function of fibroblastic V79 cells exposed to mercury chloride and 1,3,5-trinitrobenzene (TNB). Attachment, spreading and proliferation of V79 fibroblastic cells cultured on a microarray of small gold electrodes precoated with fibronectin ...

Varicella-Zoster virus (VZV) is a human herpesvirus that causes varicella (chickenpox) as primary infection, and, following variable period of ganglionic latency in neurons, it reactivates to cause herpes zoster (shingles). An analysis VZV infection cultures neural cells, particular when these have been obtained from induced pluripotent stem cells (iPSCs) or consisting highly purified neuronal ...

We have established and characterized a spontaneously immortalized, nontumorigenic mouse mammary cell line, designated IM-2. IM-2 cells synthesize large amounts of the milk protein beta-casein upon addition of lactogenic hormones. The induction of beta-casein occurs rapidly and does not require any exogenous extracellular matrix components. The IM-2 cell line is morphologically heterogeneous an...

The connective tissue of any organ in the body is generally referred to as stroma. This complex network is commonly composed of leukocytes, extracellular matrix components, mesenchymal cells, and a collection of nerves, blood, and lymphoid vessels. Once viewed primarily as a structural entity, stromal cells of mesenchymal origin are now being intensely examined for their ability to directly reg...

background: it is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (mscs). the aim of the present study was to examine whether or not they are mscs, or are distinct stem cells population residing in tooth pulp.   methods: adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through ...

background: human dental stem cells have high proliferative potential for self-renewal that is important to the regenerative capacity of the tissue. objective: the aim was to isolate human dental pulp stem cells (dpsc), periodontal ligament stem cells (pdlsc) and periapical follicle stem cells (pafsc) for their potential role in tissue regeneration. materials and methods: in this experimental s...

Human mesothelial cells obtained from benign effusions retain their proliferative capacity and grow uniformly either with a fibroblastic or epithelioid morphology in vitro. These cultures therefore provide a model for the process of mesothelial differentiation in vivo. To study this differentiation, we isolated differentially expressed genes obtained by suppression subtractive hybridization. Of...